AKLIMATISASI ANGGREK HITAM (Coelogyne pandurata Lindl.) HASIL PERBANYAKAN IN VITRO PADA MEDIA BERBEDA

Abstract

Black orchid (Coelogyne pandurata Lindl.) is an orchid endemic to the island of
Borneo. However, its existence is increasingly threatened with extinction. Conventional
propagation efforts require a long time. Therefore in vitro propagation was performed. The
purpose of this study was to determine the growth response of black orchids on the media and
different planting techniques. Black orchid plantlets that have been sub-cultured was
acclimatized in four different media types ie moss, fern, wood charcoal, wood charcoal and
coconut fiber mixture and different planting techniques namely compot (community pot) and
the individual, were allowed to grow for three months. Randomized block design (RBD) was
utilised, obtained 8 combination treatments, with 7 replicates. The results showed black
orchid has a good growth response in the media moss, ferns, and a mixture of wood charcoal
and coconut fiber, while the wood charcoal media showed unfavorable results. Different
planting techniques showed no significant results. Percentage of high life shown in mixed
media wood charcoal and coconut fiber and compot techniques.
Keyword : Black orchid (Coelogyne pandurata Lindl.), acclimatization, media