PENGARUH KOMBINASI JENIS PELARUT DAN PERBANDINGANNYA TERHADAP KARAKTERISTIK EKSTRAK BUAH PANDAN (Pandanus tectorius)
Pandan plant (Pandanus tectorius) grow well at the coast land of many tropical countries.
The ripe fruit of the pandan plant has an oval shape, red color, and contain bioactive compounds,
such as carotenoid. Extraction process is required to find the natural extract of the fruit. The aims of
this study was to find out the effect of the solvent type combination and their ratio on the
characteristics of pandanus fruit extract. This study was experimental study, which was designed by
randomized block design. The experiment was conducted as a factorial experiment, which consisted
of two factors. The first factor was the solvent type combination (acetone:chloroform and
acetone:ethanol) and the second factor was the ratio of solvent (1:1; 1:2; and 1:3). The best solvent
combination and its ratio for producing the extract was determined by effectiveness test. The results
showed that the solvent type combination affected the yield, the total carotenoids content, the level
of brightness (L*), and the level of redness (a*) but did not affect the level of yellowness (b*). The
ratio of solvent combination affected the yield but did not affect the total carotenoid content, the
level of brightness (L*), the level of redness (a*), and the level of yellowness (b*). The combination
of acetone and ethanol with ratio of 1:3 was the best treatment to produce pandanus fruit extract
with the yield of 3.13%; total carotenoid content of 12.72%; the level of brightness (L*) 11.11; the
level of redness (a*) 3.81; and the level of yellowness (b*) 31.48.
Keywords: a combination of solvents, solvent ratio, pandanus tectorius, extraction
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