STUDI PENDAHULUAN DERIVATISASI BEBERAPA SENYAWA DENGAN GUGUS AMIN MENGGUNAKAN DANSIL KLORIDA PADA KLT-SPEKTROFOTODENSITOMETER

  • Dewi Puspita Apsari
  • Ni Putu Linda Laksmiani
  • I.N.K. Widjaja

Abstract

Advance derivatization study of several amines with dansyl chloride has been carried out. The aim of this study was to determine influence of molecul structures compound toward dansylation. Derivatization was done by reacting  4000 ng drugs which contain primary or secondary amine group with 100 ng dansyl chloride at a temperature 450 C and pH 9 for a hour. Interference was separated using stationary phase silica G60 and mobile phase  etyl acetat : methanol : ammonia (85:10:5), detection on spectrophotodensytometer ?eks 312 nm mode fluorescence with filter K400.

Compounds that have a primary or secondary amine groups can be derivatized using  dansyl chloride though that most affects  of  the reaction is  rigidity of molecul structure. Differences fluorescence intensity between each derivatives influenced by the reactivity amine groups and steric hindrance of the compound.

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Author Biographies

Dewi Puspita Apsari
Mahasiswa Jurusan Farmasi Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Udayana
Ni Putu Linda Laksmiani
Jurusan Farmasi Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Udayana
I.N.K. Widjaja
Jurusan Farmasi Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Udayana
How to Cite
APSARI, Dewi Puspita; LAKSMIANI, Ni Putu Linda; WIDJAJA, I.N.K.. STUDI PENDAHULUAN DERIVATISASI BEBERAPA SENYAWA DENGAN GUGUS AMIN MENGGUNAKAN DANSIL KLORIDA PADA KLT-SPEKTROFOTODENSITOMETER. Jurnal Farmasi Udayana, [S.l.], mar. 2013. ISSN 2622-4607. Available at: <https://ojs.unud.ac.id/index.php/jfu/article/view/5193>. Date accessed: 05 nov. 2024.
Section
Articles

Keywords

amine group, dansyl chlorida, derivatization, molecul structures, TLC-spectrophotodensytometer