GROSS PATHOLOGY AND HISTOPATHOLOGY DESCRIPTION OF IMMUNE ORGANS OF BALI DUCK AFTER EXPERIMENTAL INFECTION WITH AVIAN ORTHOAVULAVIRUS 1 TABANAN-1/ARP/2017 ISOLATE
Abstract
This research aims to determine the gross pathological and histopathological changes of the thymus, bursa of Fabricius, and spleen of bali ducks infected with newcastle disease virus or Avian orthoavulavirus 1 Tabanan-1/ARP/2017 isolate. This research used 15 male bali ducks aged one week. After the acclimatization period for two weeks and the measurement of ND antibody titer in duck serum showed results below 23 HI units, 10 ducks were infected with AOAV-1 Tabanan-1/ARP/2017 isolate at a dose of 0.5 ml/27 HA units intraocularly (P1) and 5 other ducks as the control (P0) were given 0.5 ml of phosphate buffered saline by the same route. Then necropsy was performed on the dead ducks and gross pathology examination was performed on its immune organs. That organs were processed and stained for Hematoxylin-Eosin, then performed histopathology examination. The results showed that infection with AOAV-1 Tabanan-1/ARP/2017 isolate in bali ducks could cause gross pathological changes in the form of necrotic foci lesions in the spleen and atrophy in the thymus. Meanwhile, the histopathological appearance showed severe depletion of lymphoid cells in the thymus and bursa of Fabricius, and mild lymphoid cell depletion in the spleen.
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References
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Adi AAAM, Astawa NM, Putra KSA, Hayashi Y, Matsumoto Y. 2010. Isolation and characterization of a pathogenic newcastle disease virus from a natural case in Indonesia. J. Vet. Med. Sci. 72: 313-319.
Adi AAAM, Astawa NM, Wandia IN, Putra IGAA, Winaya IBO, Krisnandika AAK, Wijaya AAGO. 2019b. Karakteristik molekuler virus avian orthoavulavirus 1 genotipe VII yang diisolasi dari Tabanan Bali. J Vet. 20(4): 593-602.
Aguanta BN, Fuller AL, Milfort MC, Williams SM, Rekaya R, Aggrey SE. 2018. Histologic Effects of Concurrent Heat Stress and Coccidial Infection on the Lymphoid Tissues of Broiler Chickens. Avian Dis. 62: 345–350.
Anis Z, Morita T, Azuma K, Ito H, Itoand T, Shimada A. 2013. Histopathological alterations in immune organs of chickens and ducks after experimental infection with virulent 9a5b newcastle disease virus. J. Comp. Path. 149: 82-93.
Berthault C, Larcher T, Härtle S, Vautherot JF, Trapp-Fragnet L, Denesvre C. 2018. Atrophy of primary lymphoid organs induced by Marek’s disease virus during early infection is associated with increased apoptosis, inhibition of cell proliferation and a severe B-lymphopenia. Vet. Res. 49(1).
Bharathi AA, Muthusamy P, Rathnapraba S, Selvan ST, Srinivasan G. 2018. Evaluation of histopathological changes in ducks infected with newcastle disease virus. Int. J. Curr. Microbiol. App. Sci. 7(6): 3768-3774.
Dai Y, Cheng X, Liu M, Shen X, Li J, Yu S, Zou J, Ding C. 2014. Experimental infection of duck origin virulent Newcastle disease virus strain in duck. BMC Vet. Res. 10: 164.
Elbestawy AR, Ellakany HF, El-Hamid HSA, Zedan RE, Gado AR, Sedeik ME, El-Hack MEA, Saadeldin IM, Alowaimer AN, Ba-Awadh H, Swelum AA. 2019. Muscovy ducks infected with velogenic Newcastle disease virus (genotype VIId) act as carriers to infect in-contact chickens. Poult. Sci. 10: 1–8.
Etriwati, Ratih D, Handharyani E, Setyaningsinh S. 2017. Pathology and immunohistochemistry study of Newcastle disease field case in chicken in Indonesia. Vet. World. 10(9): 1066-1071.
Harrison L, Brown C, Afonso C, Zhang J, Susta L. 2011. Early occurrence of apoptosis in lymphoid tissues from chickens infected with strains of newcastle disease virus of varying virulence. J. Comp. Pathol. 145(4): 327–335.
Kang Y, Feng M, Zhao X, Dai X, Xiang B, Gao P, Li Y, Li Y, Ren T. 2016. Newcastle disease virus infection in chicken embryonic fbroblasts but not duck embryonic fbroblasts is associated with elevated host innate immune response. Virol. J. 13: 41.
Kiernan JA. 2015. Histological and Histochemical Methods: Theory and Practice. 5th Ed. United Kingdom. Scion Publishing. Pp. 330-334.
Kingston DJ, Dharsana R, Chavez ER. 1978. Isolation of mesogenic Newcastle disease virus from an acute disease in Indonesian ducks. Trop. Anim. Health. Prod. 10: 161-164.
Lu A, Diao Y, Chen H, Wang J, Ge P, Sun X, Hao D. 2014. Evaluation of histopathological changes, viral load, and immune function of domestic geese infected with Newcastle disease Virus. Avian Pathol. 43: 325–332.
Ravindra PV, Tiwari AK, Ratta B, Bais MV, Chaturvedi U, Palia SK, Sharma B, Chauhan RS. 2009. Time course of newcastle disease virus induced apoptotic pathways. Virus Res. 144: 350–54.
Rohajawati S, Supriyati R. 2010. Diagnosis penyakit unggas dengan metode certainty factor. Comm. IT. 4(1): 41-46.
Roy P, Venugopalan AT, Manvell R. 2000. Characterization of Newcastle disease virus isolated from chickens and ducks in Tamilnadu, India. Vet. Res. Comm. 24: 135-142.
Rue CA, Susta L, Cornax I, Brown CC, Kapczynski DR, Suarez DL, King DJ, Miller PJ, Afonso CL. 2011. Virulent Newcastle disease virus elicits a strong innate immune response in chickens. J. Gen. Virol. 92: 931-939.
Saepulloh M, Darminto. 2005. Kajian newcastle disease pada itik dan upaya pengendaliannya. WARTAZOA. 15(2): 84-94.
Sultan S, Osman N, Mohamed MA, Ahmed AI, Abdallah RAM, Faraouk M, Taha M, 2016. Infectious bursal disease vaccine ameliorates velogenic newcastle disease virus infection in immunopotentiated chickens. Comp. Clin. Pathol. 25(1): 91-100.
Udayana IDGA. 2012. Itik Bali Sebagai Hewan Upacara dan Produksi. Denpasar. Udayana University Press.
Zachary JF. 2012. Nervous System. In: McGavin MD, Zachary JF. (Ed.) Pathologic Basis of Veterinary Disease. 5th ed. St. Louis (US). Mosby Elsivier. Pp. 771-870.
Zahid B, Akhtar R, Ahmed QA, Akram Q, Yasmeen R, Ateeq MK, Raza M, Rizwan HM, Iqbal Z, Saleem MM, Imran M. 2021. Clinico-pathological assessment of virulent Newcastle Disease Virus in ducks. Braz. J. Biol. 84.
Published
2022-05-12
How to Cite
AL MA'ARIF, M. Farhan; ADI, Anak Agung Ayu Mirah; WINAYA, Ida Bagus Oka.
GROSS PATHOLOGY AND HISTOPATHOLOGY DESCRIPTION OF IMMUNE ORGANS OF BALI DUCK AFTER EXPERIMENTAL INFECTION WITH AVIAN ORTHOAVULAVIRUS 1 TABANAN-1/ARP/2017 ISOLATE.
Buletin Veteriner Udayana, [S.l.], p. 659-667, may 2022.
ISSN 2477-2712.
Available at: <https://ojs.unud.ac.id/index.php/buletinvet/article/view/86256>. Date accessed: 21 nov. 2024.
doi: https://doi.org/10.24843/bulvet.2022.v14.i06.p08.
Section
Articles
