• I Wayan Suardana Laboratorium Kesehatan Masyarakat Veteriner, Fakultas Kedokteran Hewan, Universitas Udayana, Jl. PB. Sudirman, Denpasar, Bali
  • Ni Made Ayu Aryati Dinarini Dokter Hewan Praktisi di Kabupaten Tabanan, Bali.
  • I Dewa Made Sukrama Laboratorium Biologi Molekuler dan Sumber Daya Genetik, Fakultas Kedokteran, Universitas Udayana, Jl. PB. Sudirman, Denpasar, Bali


Streptococcosis is a disease caused by Streptococcus sp., with clinical manifestations such as polyarthritis, septicemia, and meningitis. ?-hemolytic Streptococcus strains are known as one of the important pathogenic bacteria that cause serious illness. The bacteria enter their host through wounds, abrasions, food, or the immune system's decrease. This study aimed to determine the presence of ?-hemolytic Streptococcus spp in pigs raised on the people's farms in Punggul and Bongkasa Villages Abiansemal, Badung, Bali.  As many as 47 isolates isolated from nasal mucosa swabs and tonsils of pigs were cultured on blood agar media, continued by planted them on Brain Heart Infussion (BHI) agar. Gram staining, catalase, and oxidaase tests were performed as biochemical tests to complete the identification of isolates. Finally, the bacitracin test was conducted on several isolates characterized with ?- hemolytic Streptococcus to differentiate Streptococcus pyogenes strains from others. The results show, 8 out of 47 (17%) samples tested are presumptive ?-hemolytic Streptococcus. Furthermore, the bacitracin test clarifies 3 out of 8 (37.5%) ?-hemolytic isolates are S. pyogenes, and the remaining 5 out of 8 (62.5%) are non-pyogenes ?-hemolytic Streptococcus.


Download data is not yet available.


Anonimus. 2017. Dua Warga Badung Bali Positif Meninhitis Suis, Terkena Dari Hidangan Babi.
Bağcigil AF, İkiz S, Metiner K, Özgür NY, Ak. Seyyal, Ilgaz AA. 2013. Isolation of Streptococcus species from the tonsils of slaughtered pigs. Turk. J. Vet. Anim. Sci. 37: 94-96.
Bradley SF, Gordon JJ, Baumgartner DD, Marasco WA, Kauffman CA. 1991. Group C Streptococcal bacterimia: analysis of 88 cases. Rev. Infect. Dis. 13: 270-280.
Dharma DMN, Dartini NL, Soeharsono, Supartika E, Dibia N. 1994. Wabah streptococcal meningitis pada babi dan kera di Bali. Bull. Sain. Vet. 10(26): 110-121.
Dibia N, Soeharsono S, Dartini NL, 1995. Keganasan isolat Streptococcus zooepidemicus pada kera, mencit dan babi. Bull. Vet. 6(3): 6-12.
Higgins R, Gottschalk M. 1999. Streptococcal Diseases. In: Straw BE, Allaire S, Mengeling WL, Taylor DJ. Eds. Diseases of Swine. 8thEd, Iowa University Press, Iowa, USA Pp. 563–578.
Jawetz E, Melnick JL, Adelbreg EA. 1995. Mikrobiologi Kedokteran Edisi 20. Jakarta, EGC.
Katsumi M, Kataoka Y, Takahasmi T, Kikuchi N, Hiramune T. 1998. Biochemical and serological examination of beta-haemolytic streptococci isolated from slaughtered pigs. J. Vet. Med. Sci. 60: 129–131.
Kilian M. 1998. Streptococcus and Lactobacillus in Balows A and Duerden BI (eds) Topley and Wilson’s Microbiology and Microbial Infections, 9th edition, Vol. 2. Arnold, London Pp. 633–667.
Kilian M. 2012. Streptococcus and Enterococcus. in Greenwood D, Barrer M, Slack R, Irving W. (eds), Medical Microbiology: A Guide to Microbial Infections: Pathogenesis, Immunity, Laboratory Investigation and Control. 18thEd. Vol.1, Churchill Livingstone Elsevier, London, Pp. 183-198.
Krauss H, Weber A, Appel M, Enders B, Isenberg HD, Schiefer HG, Slenczka W, Graevenitz AV, Zahner H. 2003. Zoonoses. Infectious diseases transmissible from animals to humans. 3rd Ed. ASM Press.
Lindahl G, Stalhammar-Carlemalm M, Areschoug T. 2005. Surface proteins of Streptococcus agalactiae and related proteins in other bacterial pathogens. Clin. Microbiol. 18: 102-127.
Malhotra-Kumar S, Wang S, Lammens C, Chapelle S, Goossens H. 2003. Bacitracin-resistant clone of Streptococcus pyogenes isolated from pharyngitis patients in Belgium. J. Clin. Microbiol. 41(11): 5282–5284
MacFaddin JF. 2000. Biochemical Tests for Identification of Medical Bacteria. 3rdEd, Lippincott Williams & Wilkins, Philadelphia.
Patterson MJ. 1996. Streptococcosis in medical microbiology, 4 Ed.
Shabayek S, Spellerberg B. 2018. Group B streptococcal colonization, molecular characteristics, and epidemiology. Front. Microbiol. 9: 437.
Sihombing DTH. 2006. Ilmu Ternak Babi. Cetakan Kedua. Gadjah Mada University Press. Yogyakarta. Pp. 201-392: 472-484.
Suardana IW, Pinatih KJP, Ratnawati NLKA, Widiasih DA. 2013a. Protein profile analysis of Escherichia coli O157:H7 from human and animals origin. Int. J. Curr. Microbiol. App. Sci. 2(6): 204-214.
Suardana IW, Artama IWT, Widiasih DA, Mahardika IGNK. 2013b. Genetic diversity of Escherichia coli O157:H7 strains using random amplified polymorphic DNA (RAPD). Int. Res. J. Microbiol. 4(2): 72-78.
Suardana IW. 2014. Analysis of nucleotide sequences of the 16S rRNA gene of novel Escherichia coli strains isolated from feces of human and bali cattle. J. Nucleic Acids. 2014(475754): 1-7.
Suardana IW, Widiasih DA, Mahardika INK, Pnatih KJP, Daryono BS. 2015. Evaluation of zoonotic potency of Escherichia coli O157:H7 through arbitrarily primed PCR methods. Asian. Pac. J. Trop. Biomed. 5(11): 915-920.
Tran TT, Palmer HR, Weimar MR, Arias CA, Cook GM, Murray BE. 2015. Oral bacitracin: a consideration for suppression of intestinal vancomycin-resistant enterococci (vre) and for vre bacteremia from an apparent gastrointestinal tract source. Clin Infect Dis. 60(11): 1726–1728.
Todar K. 2012. Streptococcus pyogenes and Streptococcal Disease. Kenneths’s online Textbook of Bacteriology. (Diakses Tanggal 23 Januari 2018. Tersedia dari: URL:
How to Cite
SUARDANA, I Wayan; DINARINI, Ni Made Ayu Aryati; SUKRAMA, I Dewa Made. IDENTIFICATION OF ?-HEMOLYSIS STREPTOCOCCUS SPECIES ISOLATED FROM NASAL AND TONSILLAR’SWINE USING BACITRACIN TEST. Buletin Veteriner Udayana, [S.l.], p. 27-33, feb. 2021. ISSN 2477-2712. Available at: <>. Date accessed: 23 sep. 2021. doi: