THE ADDITION OF BOVINE SERUM ALBUMIN TO BELTSVILLE THAWING SOLUTION DILUENTS AGAINST MOTILITY AND VITALITY OF LANDRACE PIGS SPERMATOZOA
Abstract
This study aims to determine the effect of the addition of various bovine serum albumin (BSA) concentrations on betsville thawing solution (BTS)® extender to maintaining vitality and motility of spermatozoa on Landrace pigs. This study used Completely Randomized Design (CRD) with four treatments namely T0 (control): were given BTS® without BSA, T1: were given BTS® added BSA 5 mg/ml, T2: were given BTS® added BSA 10 mg/ml and T3: were given BTS® added BSA 20 mg/ml. Each treatment combination was repeated five times, with the total sample used was 20 samples. The measured variables in this study were the vitality and motility of Landrace pig semen. The results showed that the addition of BSA to BTS® diluents could increase the vitality and motility of spermatozoa on Landrace pigs. Where the optimal concentration was 10 mg/ml in keeping stability and maintaining the osmosis pressure stable
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References
Agustina KK., Dharmayudha AAGO, Oka IBM, Dwinata IM, Kardena IM, Dharmawan NS, Damriyasa IM. 2016b. Case of entamoebiasis in pigs raised with a free range systems in Bali, Indonesia. J. Vet. 17: 570–575.
Agustina KK, Sari PH, Suada IK. 2017a. Pengaruh perendaman pada infusa daun salam terhadap kualitas dan daya tahan daging babi. Bul. Vet. Udayana. 9(1): 34-41.
Agustina KK, Swacita IBN, Oka IBM, Dwinata IM, Traub RJ, Cargill C, Damriyasa IM. 2017. Reducing zoonotic and internal parasite burdens in pigs using a pig confinement system. Vet. World. 10(11): 1347-1352.
Chatterjee S, De Lamirande E, Gagnon. 2001. Cryopreservation alters membrane sulfhydryl status of bull spermatozoa: Protection by oxidized glutathione. Mol. Reprod. Dev. 60: 498-506.
Friedli GL. 2006. Interaction Of SWP With Bovine Serum Albumin (BSA) http://www.friedli.com/research/PhD/ chapter5.html (Tanggal akses 20 April 2011)
Gadea J. 2003. Pig industry-semen extenders used in the artificial insemination of swine. A review. Spanish J. Agric. Res. 1(27): 17-27.
Glossop CE. 2001. AI and its influenceon production efficiency, pork-production agricultural and food. Malmesbury, Wiltshire, England.
Kariyasa. 2016. Analisis penawaran dan permintaan serta potensi ekspor daging babi di Indonesia. http://media.neliti.com/media/publications/43906-ID-analisis-penawaran- dan-permintaan=daging-sapi-di-indonesia-sebelum-dan-saat-kris.pdf (Tanggal akses 2 April 2018)
Paulenz H, Kommisrud E, Hofmo PO. 2000. Effect of long-term storage at different temperatures on the quality of liquid boar semen. Repord. Dom. Anim. 35: 83-85.
The´rien I, Moreau R, Manjunath P. 1999. Major proteins of bovine seminal plasma andhigh-density lipoprotein Induce cholesterolefûux from epididymal sperm. Biol. Reprod. 59: 768-776.
