EFFECT OF VIROTHERAPY OF NEWCASTLE DISEASE VIRUS ISOLATE GIANYAR-1/AK/2014 ON SPLEEN HISTOPATHOLOGY OF WHITE RAT FIBROSARCOMA MODEL
Abstract
Fibrosarcoma is cancer that originates from fibroblast cells that grow uncontrollably and indefinitely. Surgery, chemotherapy, and radiotherapy are commonly used anticancer therapies, but still have a negative effect on normal body cells, so alternative therapies are carried out using replication of oncolytic viruses, namely Newcastle disease virus. The purpose of this study was to determine the damage or negative effects on the spleen organ after virotherapy using Newcastle disease virus by observing the histopathological picture. This study used 12 rats that had been induced benzo(a)pyrene, after macroscopically observed cancer with a diameter of 3-4 mm, 6 tails with almost uniform cancer size. Grouped into two treatments, namely the control group (P0) injected with Phosphate Buffer Saline (PBS) amounting to 0.5 mL and the treatment group (P1) injected with Newcastle disease virus at a dose of 0.5 mL/28 HA units. The variables observed were congestion lesions, hemorrhage, and necrosis. The results of histopathological observations from both groups showed no specific damage to the spleen organ, but lymphocyte proliferation was observed. Based on these results, it can be concluded that virotherapy with Newcastle disease virus isolate Gianyar-1/AK/2014 has no effect on the histopathology of the spleen of white rats with fibrosarcoma.
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References
Dennis MM, McSporran KD, Bacon NJ, Schulman FY, Foster RA, Powers BE. 2011. Prognostic factors for cutaneous and subcutaneous soft tissue sarcomas in dogs. Vet. Pathol. 48(1): 73–84.
Fournier P, Schirrmacher V. 2013. Oncolytic newcastle disease virus as cutting edge between tumor and host. Biology. 2(3): 936–975.
Junqueira LC. 2010. Histologi Dasar. 12th ed. Jakarta: Penerbit Buku Kedokteran EGC.
Kiernan J. 2015. Histological and Histochemical Methods: Theory and Practice. 5th ed. Scion Publishing Banbury.
Leary S, Underwood W, Anthony R, Cartner S, Corey D, Grandin T, Greenacre CB, Gwaltney-Bran S, McCrackin MA, Meyer R, Miller D, Shearer J, Turner T, Yanong R. 2020. AVMA Guidelines for the Euthanasia of Animals. In American Veterinary Medical Association: Schaumburg, IL, USA. (Vols. 2020–1, p. 60). American Veterinary Medical Association, Schaumburg, IL, USA.
Lutviana A, Adi AAAM, Winaya IBO. 2022. Viroterapi virus penyakit tetelo pada tikus penderita fibrosarkoma tidak berpengaruh terhadap histopatologi limpa. Indon. Med. Vet. 11(2): 147–158.
Motalleb G. 2013. Virotherapy in cancer. Iran. J. Cancer Prev. 6(2): 101–107.
Pradnyandika I, Astawa I, Adi A. 2023. Newcastle disease virus as virotherapy agent targeting p53 in rat fibrosarkoma models. J. Adv. Vet. Res. 13(1): 65–69.
Prakoeswa, FR. 2020. Peranan sel limfosit dalam imunulogi: artikel review. J. Sains Kes. 2(4): 525–537.
Rakhmawati I, Adi AAAM, Winaya IBO, Sewoyo PS. 2022. In vivo oncolytic potency of newcastle disease virus Gianyar-1/AK/2014 virulent strain against mice fibrosarcoma models. Rev. Elect. Vet. 23(1): 56–63.
Setiawan A. 2015. Pengaruh ekstrak etanol akar widuri (calotropis gigantea l.) terhadap kadar ifn-y dan ekspresi caspase 3 pada fibrosarcoma mencit (mus musculus l.). Dissertations/Thesis. Malang: Universitas Islam Negeri Maulana Malik Ibrahim.
Sewoyo PS, Adi AAAM, Winaya IBO, Sampurna IP, Bramardipa AAB. 2021. The potency of virulent newcastle disease virus Tabanan-1/ARP/2017 as virotherapy agents in rat fibrosarcoma models. J. Adv. Vet. Res. 11(2): 64–68.
Siwi RM, Prasetyorini H. 2019. Pendidikan kesehatan melalui media booklet terhadap peningkatan perawatan diri pasien kanker dengan kemoterapi. J. Manaj. Asuhan Kep. 3(1): 2685–1946.
Sofyan MS, Supranianondo K, Yudaniayanti IS, Novasari A, Paraswati E. 2020. Fibrosarcoma pada anjing golden retriever di rumah sakit hewan pendidikan universitas airlangga. ARSHI Vet. Letters. 4(2): 33–34.
Song H, Zhong LP, He J, Huang Y, Zhao YX. 2019. Application of newcastle disease virus in the treatment of colorectal cancer. World J. Clin. Cases. 7(16): 2143–2154.
Sucitra DA. (2018). Ekstrak propolis sebagai preventif infeksi virus ND (newcastle disease) secara in vitro pada kultur makrofag berdasarkan penurunan interferon alpha (Ifn-Α) dan interleukin-17 (Il-17). Dissertations/Thesis. Malang: Universitas Brawijaya.
Sukardiman NIDN, Balqianur T. 2015. The role of ethyl acetate fraction of andrographis paniculata and doxorubicin combination toward the increase of apoptosis and decrease of VEGF protein expression of mice fibrosarcoma cells. Int. J. Pharm. Pharmaceutical Sci. 7(4): 347–350.
Tayeb S, Zakay-Rones Z, Panet A. 2015. Therapeutic potential of oncolytic Newcastle disease virus: a critical review. Oncol. Virother. 4: 49-62
Vascellari M, Baioni E, Ru G, Carminato A, Mutinelli F. 2009. Animal tumour registry of two provinces in northern Italy: incidence of spontaneous tumours in dogs and cats. BMC Vet. Res. 5(1): 1–9.
Wijaya AAGO, Adi AAAM, Winaya IBO. 2018. Perubahan histopatologi embrio ayam pasca inokulasi dengan avian paramyxovirus tipe-1 isolat G1/AK/2014. Indon. Med. Vet. 7(4): 2477–6637.
Yurchenko KS, Zhou P, Kovner AV, Zavjalov EL, Shestopalova LV, Shestopalov AM. 2018. Oncolytic effect of wild-type Newcastle disease virus isolates in cancer cell lines in vitro and in vivo on xenograft model. PLoS One. 13(4).
