Analysis of the Spectrum Pattern of Human Blood Plasma Using Raman Spectroscopy

  • Anak Agung Intan Rahmasari Udayana University
  • Ida Bagus Made Suryatika Udayana University
  • I Wayan Supardi Udayana University

Abstract

A research has been carried out on the analysis of the spectrum pattern of human blood plasma using Raman spectroscopy. The research was conducted at the Forensic, Science and Criminology Laboratory. Blood plasma samples were obtained from blood donors at the Indonesian Red Cross Blood Donor Unit, Sanglah Central General Hospital, Denpasar. This study aims to determine the spectrum pattern and obtain typical values ??of the wavenumber and intensity of human blood plasma. The method used is direct irradiation of blood samples with Raman spectroscopy using a power level of 400 mW and an exposure time of 3000 ms. The results showed that blood types A and B had one distinctive peak at wavenumber of 279.365 cm-1, blood types O and AB had three distinctive peaks, e.g. at wavenumbers 219.114, 259.375, and 279.365 cm-1. The maximum intensity value of the four different blood groups, namely blood type A is 1017.456 a.u, blood group B is 1006.857 a.u, blood group O are 664.468 a.u, 666.840 a.u, 667.943 a.u, and AB blood group are 670.791 a.u, 671.670 a.u, and 669.710 a.u.

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References

[1] D. C. Pranata, Pengaruh Suhu dan Waktu Penyimpanan Sampel Darah EDTA Terhadap Pemeriksaan Kadar Hematokrit, Skripsi, Univesitas Muhammadiyah Semarang, 2016.
[2] E. G. Batrick, Forensic Analysis by Raman Spectroscopy: An Emerging Technology, France: MEDIMOND, 2002.
[3] J. M. Chalmers, H. G. M. Edwards, and M. D. Hargreaves, Infrared and Raman Spectroscopy in Forensic Science, United Kingdom: John Wiley & Sons, 2010, pp. 5-10.
[4] L. M. Moreira, L. Silveira, F. V. Santos, J. P. Lyon, R. Rocha, R. A. Zângaro, A. B. Villaverde, and M. T. T. Pacheco, Raman Spectroscopy: a Powerful Technique for Biochemical Analysis and Diagnosis Spectroscopy, vol. 22, no. 1, 2008, pp: 1–19.
[5] P. Kalantri, R. Somani, D. T. Maklhja, Raman Spectroscopy: A Potential Technique in Analysis of Pharmaceuticals Department of Pharmaceutical Chemistry, Bharati Vidyapeeth’s College of Pharmacy, Navi, Mumbai, 2010.
[6] E. Smith, and G. Dent, Modern Raman Spectroscopy – A Practical Approach, USA: John Wiley and Sons, Ltd. 2005.
[7] G. Thomson, Forensic Applications of Raman Spectroscopy, Dissertation, England: The University of Leeds, 2000.
[8] S. E. J. Bell, J. R. Beattie, J. J. McGarvey, K. L. Peters, N. M. S. Sirimutu and S. J. Speers, Development of Sampling Method for Raman Analysis of Solid Dosage Forms of Therapeutic and Illicit Drugs, Journal of Raman Spectroscopy, vol: 35, 2004, pp: 409-417.
[9] A. M. K. Enejder, T. W. Koo, J. Oh, M. Hunter, S. Sasic, and M.S. Feld, Blood analysis by Raman spectroscopy, Department of Pathologi, Beth Israel Deaconess, Medical Center, Massachusets, OPTICS LETTER, vol.27. no.22, 2002, pp: 1-3.
[10] I. B. M. Suryatika, Optimalisasi Penggunaan Metode Instrumentasi Spektroskopi Raman dalam Menganalisa Perbedaan Spektrum Jaringan Serviks Normal dan Kanker Serviks dengan Preparat Histologi di RSUP Sanglah Denpasar, Disertasi, Program Doktor, Program Studi Doktor Ilmu Kedokteran, Fakultas Kedokteran, Universitas Udayana, Denpasar, 2021.
[11] E.G. Batrick, Application of Vibrational Spectroscopy in Criminal Forensic Analysis, England: John Wiley & Sons, 2002, pp: 1-12.
[12] D. Halliday, R. Resnick, and J. Walker, Physics Jilid 1 (Penerjemah: Pantur S., Sucipto S., 1985), Jakarta: Erlangga, 1991.
[13] K.S. Krane, Fisika Modern (Penerjemah: Wosparkrik H. J., dan Sofia N., 2011) Universitas Indonesia, Jakarta: UI Press, 1992.
[14] J. R. Sipayung, Sintesa Au Nanotriangular dengan Metode Fotoreduksi Menggunakan Laser Femtosekon dan Aplikasinya untuk Deteksi Antibiotik Amoxisilin, Skripsi, Program Studi Fisika, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Sumatera Utara, Medan, 2019.
Published
2024-02-28
How to Cite
RAHMASARI, Anak Agung Intan; SURYATIKA, Ida Bagus Made; SUPARDI, I Wayan. Analysis of the Spectrum Pattern of Human Blood Plasma Using Raman Spectroscopy. BULETIN FISIKA, [S.l.], v. 25, n. 1, p. 43 – 47, feb. 2024. ISSN 2580-9733. Available at: <https://ojs.unud.ac.id/index.php/buletinfisika/article/view/89106>. Date accessed: 21 nov. 2024. doi: https://doi.org/10.24843/BF.2024.V25.i01.p06.

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