Characterization of Human Nails Samples Using FTIR (Fourier Transform Infrared) Through Chemometric Methods PCA and Clustering

  • Shinta Shaleha Juwita Fisika
  • Ni Luh Putu Trisnawati Udayana University
  • Hery Suyanto Udayana University

Abstract

Research has been carried out on the characterization of human nail samples by FTIR (Fourier Transform Infrared) using Chemometric PCA and Clustering methods. The sample used was left middle fingernail (TTK) from three people from one family (SKR) and two people who were not family (BKR). The test was carried out on TTK nail samples with a mass ratio of TTK nails with KBr 3:1. The results characterization by FTIR showed the presence of functional group O-H, C-H, C=O, N-H, C-N, P=O, C-O-C, nitrate, and nitrite in the TTK nail samples. The results of the analysis using the Chemometric PCA and Clustering methods showed that the TTK nail samples showed similarity in one family. This is based on the similarity of the types of molecules and their absorbance values. In addition, based on the results of PCA loading analysis, the wavenumber identity of the TTK nail samples were found in the range 1597-1479 cm-1.

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References

[1] T. Hartatik, Deteksi Polimorfisme DNA Sapi Aceh, Yogyakarta: Gadjah Mada University Press, 2019, pp. 12.
[2] V. Jaehuda, Ekstrasi DNA Dari Sperma Pada alat kontrasepsi Dan Kain Yang Tersimpan Sampai Dua Belas Hari, Jurnal Simbiosis, vol.1, 2013, pp. 28-39.
[3] Ahda Yuni, M. Ihra Rozi, R. Dewi Hilda, Kualitas DNA Hasil Isolasi Dari Beberapa Bagian Batang Rambut Untuk Bahan Analisis DNA Forensik, EKSAKTA, vol.1, 2013, pp. 87-93.
[4] Dachriyanus, Analisis Struktur Senyawa Organik Secara Spektroskopi, Lembaga Pengembangan Teknologi Informasi dan Komunikasi (LIPTK) Universitas Andalas, 2004, pp. 26-36.
[5] S. Toprak, F. Kahriman, Z. Dogan, et al., The Potential Of Raman Spectroscopic Methods For The Detection Of Chlorine In Human Nail Samples, Forensic Science, Medicine and Pathology, 2020, pp. 633-640.
[6] N. L. P. Trisnawati, A. Krisandi, I. G. A. Widagda, et al., A comparation of different strategies in Principle Component Analysis (PCA) algorithm for clustering human tooth surfaceusing Laser-Induced Breakdown Spectroscopy (LIBS), Journal of Physics: Conference Series, The 9th International Conference on Theoritical and Applied Physics (ICTAP), 2020, pp. 1-7.
[7] N.S. Suarnianti, Anatomi Dan Fisiologi Pada Tubuh Manusia, Yogyakarta: Indomedia Pustaka, 2016, pp. 29.
[8] S. Roussel, J. S. Preys, Lallemand, Multivarate Data Anlysis Chemometrics, Analytical For The Food industry, Claipers France, 8th Edition, vol.301, 2014.
[9] J. N. Miller, J. C. Miller, Statistic and Chemometrics For Analytical Chemistry, 6th Edition, Ashford Colour Press Ltd, Gosport UK, 2010, pp. 221-248.
[10] J. Lehtinen, Spectroscopic Studies of Human Hair, Nail, and Saliva Samples Using a Cantilever Based Photoacoustic Detection, International Journal of Thermophysics, vol. 34, no. 6, 2013, pp. 1-7.
[11] I. W. Sari, O. N. Wolfharditia, Ni Nyoman Rupiasih, et al., Pengaruh Paparan Radiasi UVC Terhadap Struktur dan Swelling Membran Kitosan Yang Terpapar Medan Magnet, Jurnal Buletin Fisika, vol. 20, no. 2, 2019, pp. 35-40.
[12] J. Coates, Encyclopedia of Analytical Chemistry: Applications Theory and Instrumentation, John Wiley & Sons, 2006, pp. 1-23.
[13] P. M. J. Barton, Forensic Investigation of Single Human Hair Fibers Using FTIR-ATR Spectroscopy And Chemometrics, Ph.D. Thesis, School of Physical and Chemical Sciences, Queensland University of Technology, Brisbane, 2011.
[14] R. M. Silverstein, G. Clayton and C. Morrill, Penyidikan Spektrometrik Senyawa Organik, Edisi ke-4, Jakarta: Erlangga, 1986, pp. 131.
[15] A. A. Damayanti, Ni Luh Putu Trisnawati, Hery Suyanto, Identifikasi Bilangan Gelombang Daun Sirih (Piper sp.) Menggunakan Metode Spektroskopi Fourier Transform Infrared (FTIR) dan Principal Component Analysis (PCA), Buletin Fisika, 2020, vol. 22, no. 2, pp. 60-66.
Published
2021-01-19
How to Cite
JUWITA, Shinta Shaleha; TRISNAWATI, Ni Luh Putu; SUYANTO, Hery. Characterization of Human Nails Samples Using FTIR (Fourier Transform Infrared) Through Chemometric Methods PCA and Clustering. BULETIN FISIKA, [S.l.], v. 22, n. 2, p. 84-90, jan. 2021. ISSN 2580-9733. Available at: <https://ojs.unud.ac.id/index.php/buletinfisika/article/view/66516>. Date accessed: 05 oct. 2024. doi: https://doi.org/10.24843/BF.2021.v22.i02.p05.

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