Effect of Long Exposure to ELF Electromagnetic Field Radiation Intensity 500 µT on Morphology and Spermatozoa Population of Balb/C Mice
Abstract
Along with the era of development, the use of Extremely Low Frequency (ELF) electromagnetic field radiation, which has a very low frequency of 0-300 Hz, is increasingly widespread. Its impact on spermatozoa remains contradictory and requires further study. Therefore, this study aims to examine the effect of long-term exposure to ELF electromagnetic field radiation with an intensity of 500 µT on the population and morphology of Balb/C mice spermatozoa. This study uses a true experimental design with a pretest-posttest control group design. There are several tools used in this research. One of them is the ELF generator as an ELF electromagnetic field generating device. The method used in sampling mice spermatozoa is eosin staining and haemocytometer. Exposure to ELF electromagnetic fields with an intensity of 500 µT was carried out intermittently, namely 15 and 60 minutes, twice a day, and given for 5 days. The results showed that exposure to the ELF electromagnetic field did not have a significant effect on the population of Balb/C mice spermatozoa, but had a significant effect on spermatozoa morphology. The effect of magnetic fields and electric fields on the population and morphology of spermatozoa differs; namely, the electric field has a more dominant effect on the population and morphology of abnormal spermatozoa, while the magnetic field has a more dominant effect on the morphology of normal spermatozoa.
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References
[2] Rahman R.A. dan Sudarti, Pengaruh Paparan Medan Magnet Extremely Low Frequency (ELF) 500 µT Terhadap Derajad Keasaman (Ph), Massa Jenis, dan Kualitas Fisik Jambu Air, Jurnal Inovasi Penelitian dan Pembelajaran Fisika (JIPPF), vol. 2, no. 2, 2021, pp. 62-66.
[3] Ratnasari, I., Sudarti, dan Yushardi, Pengaruh Paparan Medan Magnet Extremely Low Frequency (ELF) Terhadap Derajat Keasaman (pH) Susu Sapi Segar, Jurnal Pijar Mipa, vol. 16, no. 2, 2021, pp. 276-281.
[4] Ariyani, D.T., S. Najah, E. Cahayati, Sudarti, dan K. Mahmudi, Konsep Radiasi Medan Elektromagnetik ELF (Extremely Low Frequency) Oleh Peralatan Rumah Tangga, Optika: Jurnal Pendidikan Fisika, vol. 8, no. 1, 2024, pp. 147-156.
[5] Sudarti N.W., Yulihastiti, D.A., dan Suartini, N.M, Penurunan Kualitas Sperma Tikus (Rattus Novergivus) yang Diinjeksi Vitamin C Dosis Tinggi dalam Jangka Waktu Lama, Jurnal Metamorfosa, vol. 6, no. 1, 2019, pp. 7-13.
[6] Dillasamola D, Infertilitas, Lppm Universitas Andalas, Padang, 2020.
[7] Górski, R., M. Kotwicka, I. Skibińska, M. Jendraszak, dan S. Wosiński, Effect of low-frequency electric field screening on motility of human sperm, Annals of Agricultural and Environmental Medicine, vol. 27, no. 3, 2020.
[8] Mu’nisa A., O. Jumadi, M. Junda, M.W. Caronge, dan H. Hamjaya, Teknik Manajemen dan Pengelolaan Hewan Percobaan, Jurusan Biologi FMIPA UNM, Makassar, 2022.
[9] Guleken Z, Chronic Low Frequency Electromagnetic Field Exposure Before and After Neonatal Life Induces Changes On Blood Oxidative Parameters of Rat Offspring, Annals of Medical Research, vol. 28, no. 2, 2021, pp. 361-365.
[10] Muti N.D., G. Salvio, A. Ciarloni, M. Perrone, G. Tossetta, R. Lazzarini, M. Bracci, dan G. Balercia, Can Extremely Low Frequency Magnetic Field Affect Human Sperm Parameters and Male Fertility?, Tissue Cell, vol. 82, 2023.
[11] Klimek, A., A. Nowakowska, H. Kletkiewicz, J. Wyszkowska, J. Maliszewska, M. Jankowska, L. Peplowski, dan J. Rogalska, Bidirectional Effect of Repeated Exposure to Extremely Low-Frequency Electromagnetic Field (50 Hz) of 1 and 7 mT on Oxidative/Antioxidative Status in Rat’s Brain: The Prediction for the Vulnerability to Diseases, Oxidative Medicine and Cellular Longevity, vol. 1, no. 14, 2022.
[12] Hindiyati S. H., Sudarti, dan Bektiarso S, Korelasi Bobot Tubuh Terhadap Detak Jantung Mencit Balb-C yang Dipapar Medan Magnet Extremely Low Freqeuncy (ELF) Intensitas 100 µT, Jurnal Ilmu Fisika Dan Pembelajarannya, vol. 7, no. 1, 2022, pp. 34–38.
[13] Chen Y, Braun BJ, Menger MM, Ronniger M, Falldorf K, Histing T, Nussler AK, Ehnert S, Intermittent Exposure to a 16 Hz Extremely Low Frequency Pulsed Electromagnetic Field Promotes Osteogenesis In Vitro through Activating Piezo 1-Induced Ca(2+) Influx in Osteoprogenitor Cells, J Funct Biomater, vol. 14, no. 3, 2023, pp. 165.
[14] Andika, L. W. G. dan N. R. Shafriani, Paparan Radiasi Gelombang Elektromagnetik terhadap Kualitas Sperma: Tinjauan Literatur dalam Konteks Pendidikan Kesehatan, Edukatif: Jurnal Ilmu Pendidikan, vol. 6, no. 5, 2024, pp. 6252-6260.
[15] Mutiarahmi C.N., T. Hartady, dan R. Lesmana, Kajian Pustaka: Penggunaan Mencit Sebagai Hewan Coba di Laboratorium yang Mengacu pada Prinsip Kesejahteraan Hewan, Indonesia Medicus Veterinus, vol. 10, no. 1, 2021, pp. 134-145.
[16] Sinuraya W.T., A.A. Siahaan, dan Sudarti, Potensi Pemanfaatan Radiasi Medan Elektromagnetik Extremely Low Frequency untuk Terapi Kesehatan Tulang, Jurnal Penelitian Inovatif (JUPIN), vol. 3, no. 3, 2023, pp. 597-604.
[17] Doust S.K., M. Darabyan, M. Sisakht, G. Haddadi, N. Sotoudeh, M. Haghani, dan S.M.J. Mortazavi, Extremely Low Frequency-Electromagnetic Fields (ELF-EMF) Can Decrease Spermaocyte Count And Motility And Change Testicular Tissue, Journal of Biomedical Physics dan Engineering, vol. 13, no. 2, 2023, pp. 135-146.
[18] Kasmeri, R. dan Putri A, Pengaruh Ekstrak Pedada Merah (Sonneratia Careolaris L.) Terhadap Jumlah dan Morfologi Spermatozoa Mencit (Mus Musculus L.), Jurnal Biologi dan Pendidikan Biologi, vol 6, no. 1, 2020, pp. 7-13.
