https://ojs.unud.ac.id/index.php/jhsm/issue/feedJournal of Health Sciences and Medicine2019-06-24T04:19:09+00:00Sagung Chandra Yowanijhsm@unud.ac.idOpen Journal Systems<p>Journal of Health Sciences and Medicine is a peer-reveiwed, open access international online journal. The journal was initiated to encourage the research in the field of health sciences and medicine. We publish innovative original research papers, case study, review articles and short communications dealing with all the medical specialties like Anatomy, Physiology, Biochemistry, Pharmacology, Pathology, Forensic Medicine, Microbiology, Community Medicine, Ophthalmology, Internal Medicine, General Surgery, Paediatrics, Obstetrics and Gynecology, Orthopedics, Psychiatry, Radiology, Pulmonary Medicine, Dermatology and Veneral Diseases, Infectious Diseases, Anaesthesia, Cardiology, Cancer Research, Endrocinology, Urology, Neurosurgery, Geriatric Medicine, Gastroenterology, Neurology, Nephrology, Dentistry and Medical Education. We also accept research articles in the field of Immunology, Infertility, Hematology, Medical Genetics, Laboratory Medicine, Medical Statistics, Clinical Psychology, Clinical Pharmacy, Pharmaceutical Sciences and Biotechnology.</p> <p><a href="https://scholar.google.co.id/citations?hl=id&btnA=1&user=dx23CuQAAAAJ"><img src="http://nps.unud.ac.id/assets/uploads/google_scholar_logo.JPG" alt=""></a> <img src="https://ojsrepo.unud.ac.id/assets/images/logo-doaj-343x63.jpg" alt=""> <img src="https://ojsrepo.unud.ac.id/assets/images/sinta-logo-400x143.png" alt=""></p> <p> </p> <p> </p>https://ojs.unud.ac.id/index.php/jhsm/article/view/47750The Extraction and Phytochemical Test of Toxic Extract of Xestospongia testudinaria Sponge2019-06-24T04:19:09+00:00I Made Diraswantaradira_swantara@unud.ac.idWiwik Susanah Ritasusanah.rita@unud.ac.id<p class="p1">This research aims toextraction and phytochemical test of the toxic extract of the Xestospongia testudinaria sponge</p> <p class="p1">collected from Sanur beach, Bali, Indonesia. Extraction of the sponges was carried out by methanol at room temperature.</p> <p class="p1">Toxicity screening test was done based on Bhrine Shrimp Lethality Test (BSLT). The compounds of the toxic extract were</p> <p class="p1">performed by phytochemical test. Based on the results, it was found that the methanol extract of X. testudinaria sponges has</p> <p class="p1">toxic activity with LC<span class="s1">50 </span>of 31.62 ppm. The toxic extract contained alkaloid, steroid, polyphenol, and saponin compounds.</p>2019-03-29T00:00:00+00:00##submission.copyrightStatement##https://ojs.unud.ac.id/index.php/jhsm/article/view/47788 IN SILICO PRIMER DESIGN AND ANNEALING TEMPERATURE OPTIMIZATION TO AMPLIFY THE FRAGMENT OF gyrB GENE Mycobacterium tuberculosis ISOLATE P010 USING POLYMERASE CHAIN REACTION2019-03-31T12:23:19+00:00Putu Irma Handayanihandayaniirma7@gmail.com<p class="p1">One of the factors causing XDR-TB is due to mutations in the Mycobacterium tuberculosis gene, one of them is in</p> <p class="p1">the gyrB gene. Amplification of gyrB gene fragments from Mycobacterium tuberculosis DNA using Polymerase</p> <p class="p1">Chain Reaction (PCR) method. The amplification process by the PCR method requires a pair of primers (forward</p> <p class="p1">and reverse) to limit the area to be amplified. The current study aims to obtain the best primer pair generated by in</p> <p class="p1">silico design using Clone Manager Suite 6 program while simultaneously optimizing the annealing temperature to</p> <p class="p1">amplify the fragment of gyrB Mycobacterium tuberculosis. The template used in designing the primer is the</p> <p class="p1">sequence of gyrB Mycobacterium tuberculosis H37Rv isolate obtained from NCBI database of genbank code</p> <p class="p1">AL123456.3.</p> <p class="p1">The current study obtained a pair of primer which respectively had 19 oligonucleotide length and the best annealing</p> <p class="p1">temperature of 56ºC. The primer is be able to do in silico amplification of the fragment of gyrB Mycobacterium</p> <p class="p1">tuberculosis gene isolate P010 in the nucleotide area range from 1271-1755 bp with 485 bp fragment length.</p>2018-02-02T00:00:00+00:00##submission.copyrightStatement##https://ojs.unud.ac.id/index.php/jhsm/article/view/47789 Identification of Active Antioxidant Compounds from Ungu Leaf Ethanol Extract (Graptophyllum pictum L. Griff)2019-03-31T12:27:08+00:00Ni Luh Rustinirustini.niluh@yahoo.co.id<p class="p1">This study was conducted to determine the chemical compounds contained in ungu leaf ethanol extract that has antioxidant</p> <p class="p1">properties. Ungu leaf powder (2000 g) was extracted with 10,000 mL of 96% ethanol yielding 51.77 g thick purple-green</p> <p class="p1">viscous extract. The result of phytochemical test on ungu leaf ethanol extract showed that the extract contained flavonoid,</p> <p class="p1">saponin, phenolic and steroid, with the highest content being steroid. The separation of the ethanol extract was carried out by</p> <p class="p1">column chromatography using silica gel as a stationary phase and a mixture of n-hexane: dichloromethane: ethyl acetate (2: 1:</p> <p class="p1">1) as the mobile phase, yielding 10 fraction groups. Fraction F1 is relatively pure, then identified with UV-Vis, IR, and GCMS</p> <p class="p1">spectrophotometers. The results of identification with UV-Vis, IR, and GC-MS spectrophotometers showed that ungu leaf</p> <p class="p1">ethanol extract containing antioxidant activity contained stigmasterol compounds, namely: stigmasta-5,22-dien-3-ol and</p> <p class="p1">stigmast acid-5-en-3-ol.</p>2018-02-02T00:00:00+00:00##submission.copyrightStatement##https://ojs.unud.ac.id/index.php/jhsm/article/view/47790 DETECTING TRANSOVARIAL INFECTION IN AEDES AEGYPTI BASED ON IMMUNOCYTOCHEMICAL STREPTAVIDIN BIOTIN PEROXIDASE COMPLEX ASSAY (ISBPC) IN BALI2019-03-31T12:28:56+00:00Sang Gede Purnamasangpurnama@unud.ac.id<p class="p1">Immunocytochemical method is one of dengue virus examination alternative with affordable cost. Through</p> <p class="p1">immunocytochemical methods it will be proven that transovarial transmission of dengue virus from mosquitoes to</p> <p class="p1">their eggs and its relationship with the incidence of DHF in Bali. This study is done by the installationovitrap indoor</p> <p class="p1">and outdoor as much as 1200 points. The mosquito will be cultured for dengue virus examination using the</p> <p class="p1">Immunocytochemical-Immunomodoxidase streptavidin biotin complex (IISBC) method of head squash. The results</p> <p class="p1">of examination of larvae density were known to the ovitrap index in Denpasar (4.4), Tabanan (6.6), Gianyar (8.9).</p> <p class="p1">There is evidence of transovarial transmission from female mosquitoes to eggs using Immunocytochemical</p> <p class="p1">Technique known to its transovarial index of each city ie Denpasar (11%), Gianyar (7.14%) and Tabanan (7.14%).</p> <p class="p1">Key words: transovarial, immunocytochemical, Bali</p>2018-02-02T00:00:00+00:00##submission.copyrightStatement##https://ojs.unud.ac.id/index.php/jhsm/article/view/47791 DIFFERENCE OF SGOT AND SGPT LEVEL IN STADIUM IIB-IIIB SQUAMOUS CELL CERVICAL CANCER PATIENTS BEFORE AND AFTER CHEMOTHERAPY AT SANGLAH HOSPITAL DENPASAR2019-03-31T12:31:49+00:00R. Noviyanirini.noviyani@gmail.com<p class="p1">Objective:Cervical cancer became Indonesia’s highest prevalent gynecological cancer in 2013 and the highest</p> <p class="p1">prevalent gynecological cancer at Sanglah Hospital.Paclitaxel Carboplatin is one of the regimens used for</p> <p class="p1">cervical cancer treatment at Sanglah Hospital, Denpasar. Aside of providing therapeutic effect, this regimen also</p> <p class="p1">causes hepatotoxicity. This research was trying to determine the toxic effect of Paclitaxel Carboplatin towards</p> <p class="p1">liver function based on the difference of SGOT and SGPT levels before chemotherapy cycle I and after</p> <p class="p1">chemotherapy cycle VI. Method:This wasa prospective observational research with a study case method</p> <p class="p1">conducted from January 2017 until June 2017 at Obstetric Polyclinic of Sanglah Hospital, Denpasar.Samples’</p> <p class="p1">SGOT and SGPT level before and after chemotherapy I and VI were recorded and were then analysed with</p> <p class="p1">Shapiro-Wilk normality test. If the data were distributed normally, they would undergo tpaired test and</p> <p class="p1">Wilcoxon test at 95% confidence level if they were not distributed normally. Results: Tenpatients fulfilled the</p> <p class="p1">research criteria. There was a meaningless increase for SGOT level (p=0.575) along with a meaningless increase</p> <p class="p1">for SGPT level (p=0.074) before and after Paclitaxel Carboplatin chemotherapy cycle I and VI</p> <p class="p1">respectively.Conclusion: Research of toxic effect from Paclitaxel Carboplatin chemotherapy in 10 squamous</p> <p class="p1">cell cervical cancer patients showed a meaningless difference of both SGOT and SGPT level with the value of</p> <p class="p1">p>0.05 in stadium IIB-IIIB squamous cell cervical cancer patients before and after Paclitaxel Carboplatin</p> <p class="p1">chemotherapy cycle I and VI respectively.</p>2018-02-02T00:00:00+00:00##submission.copyrightStatement##https://ojs.unud.ac.id/index.php/jhsm/article/view/47792 CHARACTERISTICS OF AMBLIOPIA IN ANISOMETROPIC PATIENTS AT EYE POLYLINIC SANGLAH GENERAL HOSPITAL DENPASAR2019-03-31T12:34:33+00:00Ni Putu Pradita Dhriti Indriyani Sukadanpraditadhriti@gmail.com<p class="p1">The purpose of this cross sectional study was to determine the characteristics of amblyopia in anisometropic</p> <p class="p1">patients at Eye Polyclinic Sanglah General Hospital Denpasar.The data hat obtained are secondary data derived</p> <p class="p1">from medical records of refractive error in anisometropic patients on April 2016-August 2017 period, and</p> <p class="p1">involved 62 patients as research subjects based on consecutive sampling.The results of this study showed that</p> <p class="p1">from 62 anisometropic patients, there were 56 patients (90%) affected amblyopia. Based on the</p> <p class="p1">sociodemographic aspect, the highest number of patients with amblyopia in the children age group were 24</p> <p class="p1">peoples (43%), females more than males were 37 peoples (66%), the majority of patients living in Denpasar</p> <p class="p1">were 28 peoples (50%), and the students were the most type of occupationin amblyopic patients is 35 peoples</p> <p class="p1">(63%). Patients with severe anisometropia were 24 peoples (43%), moderate amblyopia at 48 eyes (43%), and</p> <p class="p1">affected on both eyes (ocular dextra sinistra) about 35 peoples (63%). The type of refractive error was found to</p> <p class="p1">be at most is astigmatism about 74 eyes (66%), and the use of spectacles in 51 people (91%).Based on this</p> <p class="p1">research, it can be concluded that subject who were affected amblyopia in anisometropic patients most at age</p> <p class="p1">children, female, living in Denpasar, and as students. Severe anisometropia, moderate amblyopia, affected on</p> <p class="p1">both eyes, astigmatism, and the uses of spectacles were found to be the most of the subject.</p>2018-02-02T00:00:00+00:00##submission.copyrightStatement##