Identification of Active Antioxidant Compounds from Ungu Leaf Ethanol Extract (Graptophyllum pictum L. Griff)

  • Ni Luh Rustini Universitas Udayana

Abstract

This study was conducted to determine the chemical compounds contained in ungu leaf ethanol extract that has antioxidant


properties. Ungu leaf powder (2000 g) was extracted with 10,000 mL of 96% ethanol yielding 51.77 g thick purple-green


viscous extract. The result of phytochemical test on ungu leaf ethanol extract showed that the extract contained flavonoid,


saponin, phenolic and steroid, with the highest content being steroid. The separation of the ethanol extract was carried out by


column chromatography using silica gel as a stationary phase and a mixture of n-hexane: dichloromethane: ethyl acetate (2: 1:


1) as the mobile phase, yielding 10 fraction groups. Fraction F1 is relatively pure, then identified with UV-Vis, IR, and GCMS


spectrophotometers. The results of identification with UV-Vis, IR, and GC-MS spectrophotometers showed that ungu leaf


ethanol extract containing antioxidant activity contained stigmasterol compounds, namely: stigmasta-5,22-dien-3-ol and


stigmast acid-5-en-3-ol.

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Published
2018-02-02
How to Cite
RUSTINI, Ni Luh. Identification of Active Antioxidant Compounds from Ungu Leaf Ethanol Extract (Graptophyllum pictum L. Griff). Journal of Health Sciences and Medicine, [S.l.], v. 2, n. 1, p. 4, feb. 2018. ISSN 2622-0555. Available at: <https://ojs.unud.ac.id/index.php/JHSM/article/view/47789>. Date accessed: 13 july 2020.