The Extraction and Phytochemical Test of Toxic Extract of Xestospongia testudinaria Sponge
Abstract
This research aims toextraction and phytochemical test of the toxic extract of the Xestospongia testudinaria sponge
collected from Sanur beach, Bali, Indonesia. Extraction of the sponges was carried out by methanol at room temperature.
Toxicity screening test was done based on Bhrine Shrimp Lethality Test (BSLT). The compounds of the toxic extract were
performed by phytochemical test. Based on the results, it was found that the methanol extract of X. testudinaria sponges has
toxic activity with LC50 of 31.62 ppm. The toxic extract contained alkaloid, steroid, polyphenol, and saponin compounds.
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References
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Rahmat, E. Meiyanto, “Cytotoxic compounds isolation
of Caliapsis sponge” Indonesian Pharmacy
Journal.18(4). 2007. pp. 183-189.
[2] A. Trianto, Ambariyanto. “Isolation of Leukemia
Anticancer Compound from Sponge Agelas nakamurai
and Haliclona sp” Faculty of Fisheries and Marine
Sciences, Diponegoro University. 2005.
[3] M. J. Garson, “The Biosynthesis of Secondary
Metabolits: Why is Important” In: Spons in Time and
Space, Proceeding 4th International Porifera
Conggress, R. W. M. Van Soest, Th. M. G. Van
Kempen and J. C Braekman (eds.) Amsterdam,
Netherland, 1994, pp. 428-429.
[4] R. J. Quinn, D. J. Tucker, “A brominated bisacetylenic
acid from the marine sponge Xestospongia
testudinaria” Tetrahedron Letters. 26(23). 1985. pp.
1671-1672.
[5] R. J. Quinn, D. J. Tucker, “Further Acetylenic Acids
from the Marine Sponge Xestospongia testudinaria” J.
Nat. Prod., 54 (1). 1991. Pp. 290–294.
[6] N. B. Pham, M. S. Butler, J. N. A. Hooper, R. W.
Moni, R. J. Quinn, “Isolation of Xestosterol Esters of
Brominated Acetylenic Fatty Acids from the Marine
Sponge Xestospongia testudinaria” J. Nat. Prod.
62(10). 1999. pp. 1439-1442.
[7] W. Jiang, D. Liu, Z. Deng, N. J. de Voogd, P. Proksch,
W. Lin, “Brominated polyunsaturated lipids and their
stereochemistry from the Chinese marine sponge
Xestospongia testudinaria” Tetrahedron, 67 (1), 2011,
pp. 58-68.
[8] O. O. Lee, P. Y. Lai, H. W. Wu, X-J. Zhou, L. Miao,
H. Wang, P. Y. Qian. “Marinobacter xestospongiae sp.
nov., isolated from the marine sponge Xestospongia
testudinaria collected from the Red Sea”. International
Journal of Systematic and Evolutionary Microbiology.
62. 2012, pp. 1980–1985.
[9] L. L. Sun, C-L. Shao, J. F. Chen, Z. Y. Guo, X. M. Fu,
M. Chen, Y-Y. Chen, R. Li, N. J. de Voogd, Z-G. She,
Y-C. Lin, C-Y. Wan, “New bisabolane
sesquiterpenoids from a marine-derived fungus
Aspergillus sp. isolated from the sponge Xestospongia
testudinaria” Bioorganic & Medicinal Chemistry
Letters. 22(3). 2012. pp. 1326-1329.
[10] X. C. Nguyen, A. Longeon, V. C. Pham,, F. Urvois,
C. Bressy, T. T. V. Trinh, H. N. Nguyen, V. M. Phan,
V. M. Chau, J. F. Briand, M. L. Bourguet-Kondracki,
“Antifouling 26,27-Cyclosterols from the Vietnamese
Marine Sponge Xestospongia testudinaria” J. Nat.
Prod.76(7), 2013, pp. 1313–1318.
[11] X. Zhou, Y. Lu, X. Lin, X; Yang, B.; Yang, X; and
Liu, Y. “Brominated aliphatic hydrocarbons and sterols
from the sponge Xestospongia testudinaria with their
bioactivities”. Chemistry and Physics of Lipids. 164:
703–706 (2011).
[12] B. N. Meyer, N. R. Ferrigni, Mclaughlin, “Brine
Shrimp: A Convenient General Bioassay for Active
Plant Constituents “Journal of Planta Medical
Research. 45, 1982, pp. 31-34.
[13] J. L. Carballo, Z. L. Hernandez Inda, P. Perez, M. D.
Gravalos, “Comparison Between Two Brine Shrimp
Assays to Detect in vitro Cytotoxicity in Marine
Natural Products, BMC Biotechnology.2, 2002, pp.
1472-6570.
[14] J. B. Harborne, “Phytochemical methods : A Guide to
Modern Techniques of Plant Analysis” London,
Chapman and Hall Ltd., 1987, pp. 33-204.
[15] P. Sadek, The HPLC Solvents Guide. Wiley
Interscience. 2002.
Published
2019-03-29
How to Cite
DIRASWANTARA, I Made; SUSANAH RITA, Wiwik.
The Extraction and Phytochemical Test of Toxic Extract of Xestospongia testudinaria Sponge.
Journal of Health Sciences and Medicine, [S.l.], v. 2, n. 1, p. 4, mar. 2019.
ISSN 2622-0555.
Available at: <https://ojs.unud.ac.id/index.php/jhsm/article/view/47750>. Date accessed: 24 apr. 2024.
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