Shiga Like Toxin 1 (STX-1) Detection From Escherichia coli O157:H7 Local Isolates
Abstract
Shiga-like toxin (Stx) otherwise known as verotoxin and verocytotoxin is a toxin produced by some strains of Escherichia coli
particularly by strain O157: H7. This toxin is an AB5 toxin type which is known to have similarities with the toxins produced
by Shigella dysenteriae . Stx from E. coli O157: H7 can be distinguished into stx 1 and stx 2. Stx1is usually associated with
most outbreaks and detrimental sporadic cases of illness in humans. In this research, we observed the titer of Shiga toxin (Stx 1
/ VT1) from local isolates isolated from cattle and human feces by using vero toxin Escherichia coli -reverse passive latex
agglutination test (VTEC-RPLA) method. The results showed local isolates KL-48 (2) of human origin, SM-25 (1) of cattle
feces origin and DS 21 (4) of beef origin positively produced VT1 2 units of titer, meanwhile the isolate SM 7 (1) was negative.
Titer of toxin Stx1 produced from local isolates was known to be lower when compared to the control isolate ATCC 43894 with
8 units of titer.
Downloads
References
Imashuku, and T. Sawada. (1999). Factory Outbreak of Escherichia
coli O157:H7 Infection in Japan. Emerg. Infect. Dis. [Online]. 5(3).
pp. 424-428. Available:
https://www.ncbi.nlm.nih.gov/pubmed/10341179
[2] S. L. Foley, S. Simjee, J. Meng, D. G. White, P. F. McDermott, and S.
Zhao. (2004, April). Evaluation of Molecular Typing Methods for
Escherichia coli O157:H7 Isolates from Cattle, Food and Humans. J.
Food Prot. [Online]. 67(4). pp. 651-657. Available:
https://www.ncbi.nlm.nih.gov/pubmed/15083714
[3] H. N. Atalla, R. Johnson, S. McEwen, R. W. Usborne, and C. L. Gyles.
(2000, September). Use of Shiga Toxin (Stx) Enzyme Linked
Immunosorbent Assay and Immunoblot for Detection and Isolation of
Stx Producing Escherichia coli from Naturally Contaminated Beef. J.
Food Prot. [Online]. 63(9). pp. 1167-1172. Available:
https://www.ncbi.nlm.nih.gov/pubmed/10983787
[4] R. P. Johnson, R. C. Clarke, J. B. Wilson, S. C. Read, K. Rahn, S. A.
Renwick, K. A. Sandhu, D. Alves, M. A. Karmali, H. Lior, S. A.
McEwen, J. S. Spika, and C. L. Gyles. (1996, October). Growing
Concerns and Recent Outbreaks Involving non-O157:H7 Serotypes of
Verotoxigenic Escherichia coli. J. Food Prot. [Online]. 59(10). pp.
1112-1122. Available: http://jfoodprotection.org/doi/pdf/10.4315/
0362-028X-59.10.1112
[5] X. Jiang, J. Morgan, and M. P. Doyle. (2003, October). Thermal
Inactivation of Escherichia coli O157:H7 in Cow Manure Compost. J.
Food Prot. [Online]. 66(10). pp. 1771-1777. Available:
http://jfoodprotection.org/doi/pdf/10.4315/0362-028X-66.10.1771
[6] M. P. Doyle and J. L. Schoeni. (1987, October). Isolation of
Escherichia coli O157:H7 from Retail Fresh Meats and Poultry. J.
Appl. Environ. Microbiol. [Online]. 53(10). pp. 2394-2396. Available:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC204119/
[7] M. Samadpour, M. Kubler, F. C. Buck, G. A. Depavia, E. Mazengia, J.
Stewart, P. Yang, and D. Alfi. (2002, August). Prevalence of Shiga
Toxin-Producing Escherichia coli in Ground Beef and Cattle Feces
from King Country, Washington. J. Food Prot. [Online]. 65(8). pp.
1322-1325. Available:
http://jfoodprotection.org/doi/pdf/10.4315/0362-028X-65.8.1322
[8] E. Y. Bridson, The Oxoid Manual, 8thEd. Hampshire: Oxoid Limited,
1998.
[9] R. G. Steel and J. H. Torrie, Prinsip dan Prosedur Statistika. Jakarta:
PT. Gramedia Pustaka, 1995, pp. 168-266.
[10] S. M. Avery, A. Small, C. A. Reid, and S. Buncic. (2002, July). Pulsed
Field Gel Electroforesis Characterization of Shiga Toxin-Producing
Escherichia coli O157 from Hides of Cattle at Slaughter. Research
Note. J. Food Prot. [Online]. 65(7). pp. 1172-1176. Available:
http://jfoodprotection.org/doi/pdf/10.4315/0362-028X-65.7.1172
[11] J. T. LeJeune, S. T. Abedon, K. Takemura, N. P. Christie, and
Sreevatsan. (2004, August). Human Escherichia coli O157:H7
Genetic Marker in Isolates of Bovine Origin. Emerg. Infect. Dis.
[Online]. 10(8). pp. 1482-1485. Available:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3320398/
[12] P. D. Fey, R. S. Wickert, M. E. Rupp, T. J. Safranek, and S. H.
Hinrichs. (2000). Prevalence of Non-O157:H7 Toxin Producing
Escherichia coli in Diarrheal Stool Samples from Nebraska. Emerg.
Infect. Dis. [Online]. 6(5). pp. 530-533. Available:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2627960/
