UJI ANTAGONIS BAKTERI ENDOFIT TERHADAP Colletotrichum scovillei PENYEBAB PENYAKIT ANTRAKSONA PADA CABAI BESAR (Capsicum annuum L.)
Abstract
Colletotrichum scovillei is a newly discovered species causing anthracnose disease on chilli pepper (Capsicum annuum L.) in Bali. With the increasing reports of Colletotricum spp. resistance against chemical fungicide, biological control is taken as the alternatives to control this disease. In this study a total of 69 endophytic bacteria isolates was recovered from different solanaceae plants samples collected from nine regions of Bali and screened for the antagonism againstC. scovillei. From the potential 15 candidates of isolates with inhibition activity above 90%, hypersensitivity test were taken on tobacco leaf, and isolate CBCKB1 was chosen for further test. The CBCKB1 isolate was identified as Bacillus amyloliquefaciens based on molecular identification. Detached fruit bioassay proved that isolate CBCKB1 formula was effective in reducing the disease percentage and disease severity of anthracnose disease on chilli pepper.
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References
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Allu, S., Kumar, P., and Audipudi, V. 2014. Isolation, Biochemical and PGP Characterization of Endophytic Pseudomonas aeruginosa Isolated from Chilli Red Fruit Antagonistic against Chilli Anthracnose Disease. International Journal of Current Microbiology and Applied Sciences, 3(2): 318-329.
Alvindia, D.G. and Natsuaki, K.T. 2009. Biocontrol Activities of Bacillusamyloliquefaciens DGA14 Isolated from Banana Fruit Surface Against Banana CrownRot-Causing Fungi.Crop Protection,(28): 236-242.
Amaresan, N., Jayakumar, V. and Thajuddin, N. 2014. Isolation and Characterization of Endophytic Bacteria Associated with Chilli (Capsicum annuum) Grown in Coastal Agricultural Ecosystem. Indian Journal of Biotechnology,(13): 247-255.
Basha, H.T. 2011. “Biological Control of Chilli Anthracnose Pathogen, Colletotrichum capsici (Syd.) using Antagonistic Microorganism” (thesis). India: University of Mysore.
Basha, H., Hemannavar, V., Ramanujam, B., Rangeshwaran, R. and Sriram, S. 2010. Screening of Chilli Microflora and Other Biocontrol Agents for Their Antagonistic Effects on Colletotrichumspp. InfectingChillies. The Journal of Plant Protection Sciences, 2(1): 38-44.
Card, S., Johnson,L.,TeasdaleS. and Caradus, J. 2016. Deciphering Endophyte Behaviour: The Link between Endophyte Biology and Efficacious Biological ControlAgents. FEMS Microbiology Ecology, 92(2016): 1-19. DOI: 10.1093/femsec/fiw114.
Dewi, N.K.D.A. 2018. “Effectiveness of Rhizobacteria to Control Stem Rot Disease on Groundnut (Arachis hypogea L.) caused by Sclerotium roflsii”(thesis). Denpasar: Udayana University.
Direktorat Jenderal Hortikultura. 2018. Laporan Kerja Direktoran Jenderal Hortikultura TA. 2017. Diakses dari laman: http://sakip.pertanian.go.id/admin/data2/LAKIN%20HORTI%202017.pdf.
Ji, S.H., Paul, N.C., Deng, J.X., Kim, Y.S., Yun, B.S. and Yu, S.H. 2013. Biocontrol Activity of Bacillus amyloliquefaciensCNU114001 against Fungal Plant Diseases. Mycobiology, 41(4): 234-242. Available online at: http://dx.doi.org/10.5941/MYCO.2013.41.4.234.
Khalimi, K., Darmadi, A.A.K. and Suprapta, D.N. 2019. First Report on the Prevalence of Colletotrichum scovillei Associated withAnthracnose on Chili Pepper in Bali, Indonesia. International Journal of Agriculture & Biology, DOI: 10.17957/IJAB/15.1072.
Kim, J.D., Jeon, B.J., Han, J.W., Park, M.Y., Kang, S.A. and Kim, B.S. 2015. Evaluation of the Endophytic Nature of Bacillusamyloliquefaciens Strain GYL4 and Its Efficacyin the Control of Anthracnose. Pest Management Science, 72(8): 1529-1536. DOI: 10.1002/ps.4181.
Mahartha, K. 2013. “Uji Efektivitas Rizobakteri sebagai Agen Antagonis terhadap Fusarium oxysporum f.sp. capsiciPenyebab Penyakit Layu Fusarium Pada Tanaman Cabai Rawit (Capsicum frutescens L.)” (minithesis). Denpasar: Udayana University.
Mayee, C.D. and Datar, V.V. 1986.Phytopathometry, Technical. Bulletin-1 (Special Bulletin-3). India: Marathawada Agricultural University.
Mulaw, T., Wamishe, Y. and Jia, Y. 2018. Characterization and in Plant Detection of Bacteria that Cause Bacterial Panicle Blight of Rice. Research Journal of Plant Pathology, 1(1): 1-7. Available online at: http://www.imedpub.com/research-journal-of-plant-pathology/.
Oo, M.M. and Oh, S.K. 2016. Chilli Anthracnose (Colletotrichum spp.) Disease and its Management Approach. Korean Journal of Agricultural Science, 43(2):153-162. DOI: http://dx.doi.org/10.7744/kjoas.20160018.
Paul, N., Ji, S.H., Deng, J.X. and Yu, S.H. 2013. Assemblages of Endophytic Bacteria in Chilli Pepper (Capsicum annuum L.) and Their Antifungal Activity against Phytopathogens In Vitro. Plant Omics Journal, 6(6): 441448.
Ramanujam, B., Hemannavar, V., Basha, H. and Rangeshwaran, R. 2012. Post Harvest Fruit Bioassay of Phylloplane, Pomoplane and Endophytic MicrobesagainstChilli Anthracnose Pathogen, Colletotrichum capsici (Syd.) E. J. Butler &Bisby. Journal of Biological Control, 26(1): 62-69.
Shahzad, R., Khan, A.L., Bilal, S., Asaf, S. and Lee, I.J. 2017. Plant GrowthPromoting EndophyticBacteria versus Pathogenic Infections: an Example of Bacillus amyloliquefaciensRWL-1 and Fusarium oxysporum f. sp.lycopersici in Tomato. PeerJ,(5):1-21. DOI: 10.7717/peerj.3107.
Srikhong, P., Lertmongkonthum, K., Sowanpreecha, R. and Rerngsamran, P. 2018. Bacillus sp. Strain M10 as a Potential Biocontrol AgentProtecting Chili Pepper and Tomato Fruits from AnthracnoseDisease caused by Colletotrichum capsici. BioControl, 63(6): 833-842. Available online at:https://doi.org/10.1007/s10526-018-9902-8.
Suryadi, Y., Priyatno, T.P., Samudra, I.A., Susilowati,D.N., Patricia and Irawati, W. 2013. Karakterisasi dan Identifikasi Isolat Bakteri Endofitik Penghambat Jamur Patogen Padi. Bulletin Plasma Nutfah, 19(1): 25-32.
Widodo and Hidayat, H. 2018. Identification of Colletotrichum Species Associated with Chili Anthracnose in Indonesia by Morphological Characteristics and Species-Specific Primers. Asian Journal of Plant Pathology, 12(1): 7-15. DOI: 10.3923/ajppaj.2018.7.15.
Published
2019-11-19
How to Cite
WIBAWA, I Gede Karta Satria; SUPRAPTA, Dewa Ngurah; KHALIMI, Khamdan.
UJI ANTAGONIS BAKTERI ENDOFIT TERHADAP Colletotrichum scovillei PENYEBAB PENYAKIT ANTRAKSONA PADA CABAI BESAR (Capsicum annuum L.).
Journal of Agricultural Science and Biotechnology, [S.l.], v. 8, n. 1, p. 31-41, nov. 2019.
ISSN 2302-0113.
Available at: <https://ojs.unud.ac.id/index.php/jasb/article/view/54628>. Date accessed: 25 apr. 2024.
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