Pemanfaatan Rizobakteri Penghasil Indole Acetic Acid Sebagai Agens Penginduksi Ketahanan Tanaman Tembakau Terhadap Cucumber Mosaic Virus (CMV)
Tobacco is a high economic value commodity, in order to meet the demand for tobacco in the market it requires good quantity and quality. One of the disease
that often causes harm to tobacco is Cucumber Mosaic Virus (CMV). The utilization of indole acetic acid (IAA)-producing rhizobacteria can be used to increase plant growth and resistance from pathogenic infection. The aim of this study was to determine the effectiveness of IAA producing rhizobacteria formula in increasing the growth of tobacco plants in greenhouses, and to determine the effectiveness of rhizobacterial formulations in increasing tobacco plant resistance
against the infection of CMV.
Identification of compounds in tobacco leaves that infected with CMV was carried out to determine the effectiveness of rhizobacteria in inducing plant resistance. Compound analysis was carried out using Gas Mass Chromatography Spectroscopy (GCMS). The effectiveness of rhizobacteria in increasing plant growth and resistance was conducted in a greenhouse. Based on the results of the study, four of IAA-producing rhizobacteria isolates were Stenotrophomonas maltophilia Sg3, Proteus mirabilis BjB17, Bacillus thuringiensis TNJbx3.3, and Bacillus cereus GR12, all the rhizobacterial isolates were effective in increasing plant height, leaf number, leaf chlorophyll content, wet weight and dry weight leaf. Based on the results of the rhizobacterial effectiveness test, all rhizobacterial isolates were effective in increasing plant resistance as indicated by a lower intensity of infection. Based on the analysis of the tobacco leaves compounds content showed that rhizobacteria S. maltophilia Sg3 and P. mirabilis BjB17 were able to induce resistance to tobacco plants by forming antiviral compounds namely 2-Naphthalene-sulfonic acid and rhizobacteria Bacillus cereus GR12 can stimulate the formation of 1-Naphthalene-sulfonic acid compounds and 2-Naphthalenesulfonic acid
in tobacco as a form of induction of resistance to CMV infection.
A’yun, K.Q., T. Hadiastono, M. Martosudiro. 2013. Pengaruh Penggunaan PGPR (Plant Growth Promoting Rhizobacteria) terhadap Intensitas TMV (Tobacco Mosaic Virus), Pertumbuhan, dan Produksi pada Tanaman Cabai Rawit (Capsicum frutescens L.). Jurnal Hama dan Penyakit Tumbuhan. 1(1).47-56
Chasanah, U. 2007. Pemanfaatan Rizobakteri Pemacu Pertumbuhan Tanaman (rppt) untuk Menginduksi Resistensi Sistemik Mentimun terhadap Zucchini Yellow Mosaic Potyvirus (zymv). Tesis; Institut pertanian Bogor
Chivasa, S., Murphy A M, M. Naylor, JP. Carr. 1997. Salicylic Acid Interferes With Tabacco Mosaic Virus Replication Via a Novel Salicylhydroxamic Acid
Sensitive Mechanism. Plant Cell 9: 547-555.
Compant, S., Duffy B, Nowak J, Clement C, and E.A Barka. 2005. Use of Plant Growth Promoting Bacteria for Biocontrol of Plant Diseases; rinciples, Mechanisms of Action, and Future Prospects. Applied and Environmental Microbiology 71:4951-4958.
Diyansah, B., Aini, L.Q., and Hadiastono, T. 2003. The effect of PGPR (plant growth promoting rhizobacteria) Pseudomonas fluorescens and Bacillus subtilis on leaf mustard plant (Brassica juncea L.) infected by TuMv (Turnip Mosaic Virus). J Trop. Plant Prot.1(1):30-38
Hiyama, M. 1952. Studies on Naphthalene Sulfonic Acid derivatives. II. Aminonaphthalene Sulfonamide derivatives; Syntheses and test for their effects on bacteria and viruses. The Kanto Districts Meeting of the Pharmaceutical Society of Japan.72: 1368-1370.
Khalimi, K. 2017. Pemanfaatan Rizobakteri sebagai Biostimulan untuk Meningkatkan Kuantitas dan Kualitas Hasil Kedelai Edamame (Glycine max L. Merrill). (Disertasi). Denpasar: Universitas Udayana.
Lucas, G.B. 1975. Disease of Tobacco. Harold E, Parker and Sons Printer; North Carolina.
Palukaitis, P., M. J. Roossinck, R. G. Dietzgen, R. I. B. Francki.1992. Cucumber mosaic virus. Adv. Virus Res. 41: 281-348.
Raupach, G.S., L. Liu, J.F. Murphy, S. Tuzun, J.W. Kloepper. 1996. Induced Systemic Resistance in Cucumber Mosaic Cucumovirus Using Plant Growth Promoting Rhizobacteria (PGPR). Plant Diseses. 80:891-895.
Rusconi, S., Mona, M., Debra, P.M., Peter, V.P., Edith, A.N., Shyam, K.S., Kevin, J.W., Marcia, S.O., Albert, T.P., James, C.J., Martin, S.H. 1996. Naththalene sulfonate polymers with CD4-blocking and anti-human immunodeficiency virus type 1 activities. Antimicrobial Agents and Chemotherapy 40 (1): 234236.
Schneider, W.L., Roossinck, M.J. 2001. Genetic diversity in RNA virus quasispecies is controlled by host-virus interactions. Journal of Virology 75 (14): 6566-6571.
Surette MA, Stunz A.V., Lara R.R, Nowak J. 2003. Bacterial Endophytes in Processing Carrots (Ducus carota L. Var. Satvus): Their Localization, Population Density, Biodiversity and Their Effects on Plant Growth.Pe. Soil. 253:381-390.
Tarantino, D., Margherita, P., Eloise, M., Romina, C., Jacques, R., Ivonne, R., Martino, B., Mario, M. 2014. Naphthalene Sulfonate Inhibitors of Human Norovirus RNA-dependent RNA Polymerase. Antiviral Research.102: 2328.
Wahyuni, W.S. 2005. Dasar -Dasar Virologi Tumbuhan. Gadjah Mada University Press. Yogyakarta.