DETEKSI SIMULTAN CMV DAN CHIVMV PENYEBAB PENYAKIT MOSAIK PADA TANAMAN CABAI DENGAN DUPLEX RT-PCR

Abstract

Detect the presence virus causes mosaic symptoms by CMV and ChiVMV infecting chili pepper plants at the same time was conducted. There needs a study that aims to detect the associations of both viruses causes mosaic symptoms by single or double infection and simplify the detection process which is necessary for a long and expensive process. The used method is a duplex RT-PCR in hopes of simultaneously detecting two viruses causes mosaic disease. Based on the results of the study showed that the duplex RT-PCR method can detect double infection of CMV and ChiVMV on chili plants and this method more effective to detect two viruses simultaneously in a single PCR reaction.