DNA barcoding on marine invertebrates

  • Agus Mohammad Hikam Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Islam Malang, Jl. Mayjen Haryono No. 193, Malang65144, East Java, Indonesia
  • Nurul Jadid Mubarakati Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Islam Malang, Jl. Mayjen Haryono No. 193, Malang65144, East Java, Indonesia
  • Muhammad Dailami Departemen Budidaya Perairan, Fakultas Perikanan dan Ilmu Kelautan, Universitas Brawijaya. Jl. Veteran No. 16, Malang 65145, East Java, Indonesia
  • Abdul Hamid A. Toha Jurusan Perikanan, Fakultas Perikanan dan Ilmu Kelautan, Universitas Papua. Jl. Gunung Salju, Manokwari 98314, West Papua, Indonesia

Abstract

A large number of species of marine invertebrates that have similar morphology cause misidentification very possible. Molecular species identification is important in taxonomy study accurately. This study aims to determine the identity of Aquatic Papua marine invertebrates with DNA barcoding technique using markers COI gene (Cytochrome c oxidase I). The study identified 29 individuals of marine invertebrates, which came from sea cucumbers (6 samples), lobsters (6 samples), octopus (6 samples), chitons (5 samples), and sea urchins (6 samples). Molecular identification methods consist of DNA isolation, PCR COI gene fragments, sequencing, and DNA sequence analysis using bioinformatics software. The results showed that the sea cucumber came from the Bohadschia marmorata species, the lobster came from the Panulirus versicolor species, the octopus came from the Octopus cyanea species, the chiton came from the Ischnochitonaustralis species, and the sea urchins came from the Tripneustesgratilla species. The similarity of samples and references are in the range of 84.58 to 100.00%. The disparity index, genetic distance, and phylogenetic tree supported this result.

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Published
2021-06-25
How to Cite
HIKAM, Agus Mohammad et al. DNA barcoding on marine invertebrates. Jurnal Biologi Udayana, [S.l.], v. 25, n. 1, p. 46-56, june 2021. ISSN 2599-2856. Available at: <https://ojs.unud.ac.id/index.php/BIO/article/view/70835>. Date accessed: 18 oct. 2021. doi: https://doi.org/10.24843/JBIOUNUD.2021.v25.i01.p06.