Growth of Cymbidium bicolor Lindl. plantlet during subculture with variation of media

  • Fakhri Fadhlurrohman Pratama Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro Jl. Prof. H. Soedarto, SH., Tembalang Semarang, Jawa Tengah, Indonesia 50275
  • Nintya Setiari Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro Jl. Prof. H. Soedarto, SH., Tembalang Semarang, Jawa Tengah, Indonesia 50275
  • Yulita Nurchayati Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro Jl. Prof. H. Soedarto, SH., Tembalang Semarang, Jawa Tengah, Indonesia 50275

Abstract

Cymbidium bicolor is one of the orchids in Indonesia which has beautiful and longlasting flowers, so it has potential to become an ornamental plant and has economic value. The method of propagation generally is tissue culture. One of the stages in plant tissue culture is subculture. The variation of media used at the subculture stage make an effect for growth of plantlet. Media usually can be used include Murashige and Skoog (MS), Vacin and Went (VW), and New Phalaenopsis (NP). This study aims is to analyze the variation of media during the subculture. The method used was tissue culture in subculture with media as treatment. Cymbidium bicolor planlets were subcultured into MS, VW, and NP and observed for 2 months. The parameters observed were the leaf emergence, the number of leafs, leaf length, leaf width, the number of roots, root length, and the number of shoots. This study used a completely randomized design with single factor and 15 replicates. Data were analyzed by ANOVA with 95% significance. Treatment of variation of media significantly affected all parameters. Murashige and Skoog (MS) medium was the best medium for leaf emergence (11 days), the number of leafs (4,92), leaf length (1,56 cm), leaf width (0,33 cm), the number of roots (2,67), root length (2,73 cm), and the number of shoots (4,00). Murashige and Skoog (MS) medium was the best medium and concentrations for C. bicolor growth at the subculture stage.

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References

Akter S, Nasiruddin K, Khaldun A. 2007. Organogenesis of Dendrobium Orchid Using Traditional Media and Organic Extracts. Journal of Agriculture & Rural Development 5(1&2): 30–35.
Fitri ASS, Santoso AM. 2013. Ragam Orchidaceae Epifit di Kawasan Ubalan Kediri dan Prospeknya Sebagai Modal Bioekonomi Lokal. Proceeding Biology Education Conference 11(1): 365–370
Ikenganyia E, Anikwe M, Omeje T, Adinde J. 2017. Plant Tissue Culture Regeneration and Aseptic Techniques. Asian Journal of Biotechnology and Bioresource Technology 1(3): 1–6.
Jaime A, Silva T da. 2015. Ammonium to Nitrate Ratio Affects Protocorm Like Bodies PLB Formation In vitro of Hybrid Cymbidium. Journal of Ornamental Plants (Journal of Ornamental and Horticultural Plants) 3(3): 155–160
Kasutjianingati, Irawan R. 2013. Media Alternatif Perbanyakan In Vitro Anggrek Bulan (Phalaenopsis amabilis). Jurnal Agroteknos 3(3): 184–189
Krisdianto A, Saptiningsih E, Nurchayati Y, Setiari N. 2020. Pertumbuhan Plantlet Anggrek Phalaenopsis amabilis (L.) Blume Pada Tahap Subkultur Dengan Perlakuan Jenis Media Dan Konsentrasi Pepton Berbeda. Jurnal Metamorfosa: Journal of Biological Sciences 7(2): 40–47.
Leghari SJ, Wahocho NA, Laghari GM, HafeezLaghari A, MustafaBhabhan G, HussainTalpur K, Lashari AA. 2016. Role of nitrogen for plant growth and development : a review. Advances in Environmental Biology 10(9): 209–219
Merthaningsih NIP, Yuswanti H. 2018. Induksi Kalus pada Kultur Pollen Phalaenopsis dengan Menggunakan 2,4 Diklorofenoksiasetat. Agrotrop 8(1): 47–55
Nurhidayah T, Mardhiansyah M, Mulyani D. 2017. Pengaruh Sitokinin (Kinetin) dan Auksin (2,4 D) dalam Media Induksi Murashige dan Skoog terhadap Perkembangan Eksplan Meristem Apikal Tunas Anakan Tanaman Sagu (Metroxylon sagu Rottb.). Jurnal Agrotek Tropika 6(1): 23–28
Pertamawati. 2010. Pertumbuhan Tanaman Kentang (Solanum tuberosum L.) Dalam Lingkungan Fotoautotrof secara In vitro. Jurnal Sains dan Teknologi Indonesia 12(1): 31–37
Pradhan S, Regmi T, Parmar G, Pant B. 2013. Effect of Different Media on in vitro Seed Germination and Seedling Development of Cymbidium aloifolium (L.) Sw. Nepal Journal of Science and Technology 14(1): 51–56.
Rahmah S, Rahayu T, Hayati A. 2018. Kajian penambahan bahan organik pada media tanam VW pada organogenesis anggrek Dendrobium secara in vitro. Jurnal Sains Alami 1(1): 93–103
Rodinah R, Hardarani N, Ariani HD. 2018. Modifikasi Media Dan Periode Subkultur Pada Kultur Jaringan Pisang Talas (Musa paradisiaca VAR. SAPIENTUM L.). Jurnal Hexagro 2(2): 1–6.
Setiawati T, Zahra A, Budiono R, Nurzaman M. 2018. Perbanyakan In Vitro Tanaman Kentang (Solanum tuberosum [L.] cv. Granola) dengan Penambahan META-TOPOLIN pada Media Modifikasi MS (Murashige & Skoog). Jurnal Metamorfosa: Journal of Biological Sciences 5(1): 44–50
Uche OC, Ejiofor AP, Eziuche OC. 2016. (Comparative Growth Rates of Treculia africana Decne: Embryo in Varied Strengths of Murashige and Skoog Basal Medium). International Journal of Agricultural and Biosystems Engineering 10(9): 564–567
Utami ESW, Hariyanto S, Manuhara YSW. 2017. In vitro propagation of the endangered medicinal orchid, Dendrobium lasianthera J.J.Sm through mature seed culture. Asian Pacific Journal of Tropical Biomedicine 7(5): 406–410.
Wirawan BDS, Putra ETS, Yudono P. 2016. Pengaruh Pemberian Magnesium, Boron dan Silikon terhadap Aktivitas Fisiologis, Kekuatan Struktural Jaringan Buah dan Hasil Pisang (Musa acuminata) “Raja Bulu.” Vegetalika 5(4): 1–14.
Yang GH, Yang LT, Jiang HX, Li Y, Wang P, Chen LS. 2012. Physiological impacts of magnesium-deficiency in Citrus seedlings: Photosynthesis, antioxidant system and carbohydrates. Trees - Structure and Function 26(4): 1237–1250.
Published
2021-06-25
How to Cite
PRATAMA, Fakhri Fadhlurrohman; SETIARI, Nintya; NURCHAYATI, Yulita. Growth of Cymbidium bicolor Lindl. plantlet during subculture with variation of media. Jurnal Biologi Udayana, [S.l.], v. 25, n. 1, p. 71-77, june 2021. ISSN 2599-2856. Available at: <https://ojs.unud.ac.id/index.php/bio/article/view/67898>. Date accessed: 29 mar. 2024. doi: https://doi.org/10.24843/JBIOUNUD.2021.v25.i01.p08.