Genetic variation and genetic distance of the Sakai tribe in Riau Province and the Minangkabau tribe in Pagaruyung Village, West Sumatra based on microsatellite DNA

  • Tyara Tyara Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana, Bukit Jimbaran, Bali, Indonesia – 80361
  • I Ketut Junitha Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana, Bukit Jimbaran, Bali, Indonesia – 80361
  • Ni Nyoman Wirasiti Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana, Bukit Jimbaran, Bali, Indonesia – 80361

Abstract

There are several versions can explain the origin of the Sakai tribe. Sakai people themselves believe that their ancestors came from Pagaruyung, West Sumatra Province. The purpose of this research is to determine the genetic variation and genetic distance of the Sakai tribe in Riau Province and the Minangkabau tribe in Pagaruyung Village, West Sumatra Province based on three microsatellite DNA loci (D2S1338, D16S539, and D13S317). A total of 10 alleles were found in three microsatellite loci of the Sakai tribe and 11 alleles in the Minangkabau tribe.  Data analysis using GenAlex 6.503 software showed that the heterozygous value of the Sakai and Minangkabau tribes was moderate with a value of 0.557 ± 0.062 in the Sakai tribe and 0.615 ± 0.073 in the Minangkabau tribe. Meanwhile, the value of genetic differentiation (F’ST) in these two tribes shows moderate genetic differences (0.088 ± 0.039). Genetically, Sakai people and Minangkabau tribes have similarities based on the D2S1338, D16S539, and D13S317 microsatellite DNA loci with a genetic distance of 0.269 and the proportion of identical genes (Genetic identity) of 0.764. This result is supported by alleles outflow or entry in the Sakai and Minangkabau tribes with a migration rate is four people per generation.

Downloads

Download data is not yet available.

References

Arnila GAP, Junitha IK, Pharmawati M. 2016. Variasi Genetik Masyarakat Soroh Pande di Kabupaten Gianyar Berdasarkan Tiga Lokus DNA Mikrosatelit Autosom. Jurnal Biologi 20(1): 1-5.
Agnes RS, Ahmad A. 2007. “Runtuhnya Kearifan Suku Sakai,” Bagian 2. Harian Kompas, Rabu, 25 April 2007
Bahagiawati, Utami DW, Buchori D. 2010. Pengelompokan dan Struktur Populasi Parasitoid Telur Trichogrammatoidea armigera pada Telur Helicoverpa armigera pada Jagung Berdasarkan Karakter Molekuler. Jurnal Entomologi Indonesia 7(1): 54-65.
Butler JM. 2006. Genetics and Genomics of Core Short Tandem Repeat Loci Used in Human Identity Testing. Journal Forensic Science 51(2): 253-265.
Daldjoeni N. 1991. Ras-Ras Umat Manusia. Citra Aditya Bakti: Bandung
Damayanti IAM., Junitha IK, Suaskara IBM. 2014. Variasi Genetik Soroh Brahmana Budha Di Bali Berdasarkan Penanda DNA Mikrosatelit Kromosom-Y. (Skripsi). Jurusan Biologi. Fakultas Matematika dan Ilmu Pengetahuan Alam. Universitas Udayana. Bali.
Dwitiari MC. 2012. Variasi Genetik Masyarakat Bali Mula di Desa Sembiran Buleleng dengan Penanda DNA Mikrosatelit. Jurnal Biologi 16(1): 9-14.
Gonser R, Donnely P, G. Nicholson, dan A. Di Rienzo. 2000. Microsatellite Mutation and Infernces About Human Demography. Genetics 154(4): 1793- 1807.
Hutchinson F. 2001. DNA Band Size Semi-log Plotting. Cancer Research Center Science Education Partnership
Junitha IK. 2004. Keragaman Genetik Masyarakat di Desa-Desa Bali Aga Berdasarkan Analisis DNA dan Sidik Jari. Bogor. Sekolah Pascasarjana Institut Pertanian Bogor. (Disertasi). Tidak Dipublikasikan
Junitha IK, Octavia LE. 2015. Studi Pendahuluan Variasi Genetik Masyarakat Dayak di Kota Palangka Raya Kalimantan Tegnah Berdasarkan Enam Lokus Mikrosatelit Autosom. Prosiding Seminar Nasional Biosains hal. 242-247
Junitha IK, Sudirga SK. 2007. Variasi DNA Mikrosatelit Kromodom Y pada Masyarakat Bali Terunyan. Hayati Journal of Bioscience 14(2):59-64
Junitha IK, Watiniasih NL. 2014. Male Genetic Diversity of Siwa Brahmin Clan in Bali based on Y-Chromosomal Microsatellites DNA. Journal of Biology Agriculture and Healthcare 4(1): 30-35.
Junitha IK., Yossy C. 2016. Ragam Alel Dan Kekuatan Pembeda Lima Lokus Mikrosatelit Dna Autosom Masyarakat Suku Batak Di Denpasar dan Badung. 2016. Indonesian Journal of Legal and Forensic Sciences 6: 6-10.
Kurtubi D. 2017. “Mengenal Suku-Suku Asli (Komunitas Adat Terpencil) Di Provinsi Riau” tersedia dalam http://dinsos.riau.go.id/web/index.php?option=com_content&view=article&id=410:mengenal-suku-suku-asli-komunitas-adat-terpencil-di-provinsi-riau-oleh-dodi-ahmad-kurtubi&catid=17&Itemid=117 diakses pada 10 September 2018.
Laksmita AS, Junitha IK, Watiniasih NL. 2015. Struktur Genetik dan Ragam Alel Tiga Generasi Masyarakat Bali Berdasarkan Lima Lokus Penanda DNA Mikrosatelit Autosom. Metamorfosa 2(2): 58-65
Lutfi M. 1977. Sejarah Riau. Pekanbaru: Universitas Riau Press.
Maruyama S, Minaguchi K, Takezaki N, Nambiar P. 2008. Population data on 15 STR loci using AmpF/STR Identifiler kit in a Malay population living in and around Kuala Lumpur, Malaysia. Legal Medicine 10: 160–162.
Moeljopawiro S. 2010. Marka Mikrosatelit sebagai Alternatif Uji BUSS dalam Perlindungan Varietas Tanamam Padi. Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian. Buletin Plasma Nutfah 16(1):1-7
Muladno. 2000. Polimorfisme Dan Analisis Keterpautan Mikrosatelit Pada Genom Babi. Hayati 7(1): 11-15.
Mulliadi D, Johar A. 2010. Pendugaan Keseimbangan Populasi dan Heterozigositas Menggunakan Pola Protein Albumin Darah pada Populasi Domba Ekor Tipis (Javanese Thin Tailed) di Daerah Indramayu (Prediction Equilibrium of Population Used Blood Albumin Pattern of Thin Tailed Sheep Pop). Jurnal Ilmu Ternak 10(2): 65-72.
Nanasato Y, Uenoyama R, Tomooka K, Kato A, Akashi K. Nuclear isolation and purification using SDS/urea (NIPSU) method for efficient and rapid extraction of high-purity genomic DNAs from Jatropha curcas L: A comparative analysis of DNA isolation protocols. African Journal of Biotechnology 17(32): 981-988.
Nei M. 1973. Analysis of gene diversity in subdivided populations. Proceedings of the National Academy of Sciences of the United States of America 70(12): 3321-3.
Nur’aisyah. 2017. Adaptasi Suku Asli Di Desa Jangkang Kecamatan Bantan Kabupaten Bengkalis. Jom FISIP. 4 (2): 1-15.
Octavia, LE, Junitha IK, Narayani I. 2015. Variasi Alel DNA Mikrosatelit Autosom Lokus D2S1338, D13S317 Dan D16S539. Jurnal Biologi 19(2):69-73.
Pasaribu P. 2019. Manajemen Istano Basa Pagaruyung. Jurnal Pariwisata 6(1):71-79.
Peakall R, Smouse PE. 2012. GenAlEx 6.5: Genetic Analysis in Excel. Population Genetic Software for Teaching and Research - an Update. Bioinformatics 28(19): 2537–2539.
Rodriguez JJRB, Jazelyn MS, Gayvelline CC, Rita PL, Maria CADU. 2015. Allele frequencies of 23 autosomal short tandem repeat loci in the Philippine population. Legal Medicine 7(4):295-7.
Sharma R, Brijesh K, Reena A, Sonika A, Mishra AK, Tantia MS. 2016. Genetik diversity estimates point to immediate efforts for conserving the endangered Tibetan sheep of India. Meta Gene 8:14-20 .
Sheriff O, Kefyalew A. 2018. Genetik diversity studies using microsatellite markers and their contribution in supporting sustainable sheep breeding programs: A review. Cogent Food & Agriculture 4: 1459062.
Sofyan A. 2012. Sejarah Perkembangan Bahasa Sakai. Linguistika: Buletin Ilmiah Program Magister Linguistik Universitas Udayana 19 Sep 2012.
Tersedia dalam: https://ojs.unud.ac.id/index.php/linguistika/article/view/9692 diakses pada 10 September 2018.
Suparlan P. 1995. Orang Sakai di Riau: Masyarakat Terasing dalam Masyarakat Indonesia. Jakarta: Yayasan Obor.
Unadi YC, Narayani I, Junitha IK. 2010. Variasi Genetik Suku Batak yang Tinggal di Kota Denpasar dan Kabupaten Badung Berdasarkan Tiga lokus mikrosatelit DNA Autosom. Jurnal Biologi 14(2): 33-38.
Published
2021-06-25
How to Cite
TYARA, Tyara; JUNITHA, I Ketut; WIRASITI, Ni Nyoman. Genetic variation and genetic distance of the Sakai tribe in Riau Province and the Minangkabau tribe in Pagaruyung Village, West Sumatra based on microsatellite DNA. Jurnal Biologi Udayana, [S.l.], v. 25, n. 1, p. 18-27, june 2021. ISSN 2599-2856. Available at: <https://ojs.unud.ac.id/index.php/BIO/article/view/66567>. Date accessed: 25 oct. 2021. doi: https://doi.org/10.24843/JBIOUNUD.2021.v25.i01.p03.