Effect of Thiophenone Compound on Motility and Caseinase Production of Aeromonas hydrophila

  • Ayu Ashari Margareth Sinaga Udayana University
  • Pande Gde Sasmita Julyantotro Udayana University
  • Dewa Ayu Angga Pebriani Udayana University


Motility and caseinase production are part of bacterial virulence factors which are regulated by a bacterial intercellular communication system, often called quorum sensing (QS). This study aims to determine the effect of the QS inhibitor compound thiophenone in reducing those virulence factors. This research was conducted at the Laboratory of Fisheries Science, Faculty of Marine Science and Fisheries, Udayana University, from December 2019 to March 2020. This study tested 3 different treatments with 3 repetitions for each treatment. Treatment A as control (without the addition of thiophenone), treatment B (addition of 5 ?M thiophenone), and C treatment (addition of 10 ?M thiophenone). The results showed that thiophenone compounds can inhibit the QS system of Aeromonas hydrophila. It can be seen from the significantly reduced (P<0.05) motility and caseinase enzyme activity of A. hydrophila compared to without addition of thiophenone compounds. The average diameter of the caseinase enzyme production produced in treatment A at 22 hours was 21.40±0.36 mm, in treatment B was 19.70±0.2 mm and in treatment, C was 17.87±0.05 mm. Whereas in motility, the resulting average diameter in treatment A at 22 hours was 6.57±0.61 mm, in treatment B was 5.67±0.35 mm and in treatment, C was 5.10±0.6 mm. These results indicate that the QS thiophenone inhibitor compound can reduce virulence factors, namely motility and caseinase production from pathogenic bacteria A. hydrophila. Treatment C can decrease virulence factors compared to treatment A and B.


Download data is not yet available.

Author Biographies

Ayu Ashari Margareth Sinaga, Udayana University

Department of Aquatic Resources Management, Faculty of Marine and Fisheries

Pande Gde Sasmita Julyantotro, Udayana University

Department of Aquatic Resources Management, Faculty of Marine and Fisheries

Dewa Ayu Angga Pebriani, Udayana University

Department of Aquatic Resources Management, Faculty of Marine and Fisheries


[1] Chu, W., S. Zhou., W. Zhu., and X. Zhuang. 2014. Quorum Quenching Bacteria Bacillus sp. QSI-1 Protect Zebrafish (Daniorerio) From Aeromonas hydrophila Infection. Scientific Reports. 4: 5446
[2] Secades, D AND J.A. Guijarro. 1999. Purification and Characterization of an Extracelluler Proteases from Fish Pathogen Yersinia ruckeri and Effect of Cultures Condition and Production. Applied and Environmental Microbiology. 65(9): 3969-3975.
[3] Madigan, M.T., J.M. Martinko and J. Parker. 2009. Brock’s Biology of Microorganisms.9th Ed. Prentice Hall Internasional, Inc. New Jersey.
[4] Witso, I.L., T. Benneche., L.K. Vestby., L.L. Nesse., J.L. Stensrud and A.A. Scheie. 2014. Thiophenone and Furanone in The Control of Escherichia coli O103:H2 Virulence. Pathogens and Disease. 70: 297-306.
[5] Aini, N. 2006. Penurunan Produksi Enzim Eksoprotease Aeromonas Hydrophila Oleh Ekstrak Buah Tomat (Lycopersicon esculentum mill). Skripsi. Surakarta, Indonesia: Jurusan Biologi, Fakultas Matematika dan Ilmu pengetahuan Alam, Universitas Sebelas Maret.
[6] Hentzer, M., K. Riedel., T.B. Rasmussen., A. Heydorn., J.B. Anderson., M.R. Parsek., S.A. Rice., L. Eberl., S. Molin., N., Hoiby., S. Kjelleberg and M. Givskov. 2002. Inhibition of Quorum Sensing in Pseudomonas Aeruginosa Biofilm Bacteria by A Halogenated Furanone Compound. Microbiology. 148: 87-102.
[7] Kazlauskas, R.P., R. Murthy., Quin and R. Wells. 1977. A New Class of Halogenated Lactones from The Red Alga Delisea Fimbriata. Tetrahedron Letter. (1): 37-40.
[8] Yang, Q., A.A. Scheie., T. Benneche and T. Defoirdt. 2015. Specific Quorum Sensing-Disrupting Activity (AQSI) Of Thiophenone S and Their Therapeutic Potential. Sci. Rep. 5, 18033.
[9] Sudarsono, A. 2008. Isolasi dan Karakteristik Bakteri Pada Ikan Laut Dalam Spesies Ikan Gandara (Lepidocidium flavobroneum). Skripsi. Bogor, Indonesia: Fakultas Perikanan dan Ilmu Kelautan, Institiut Pertanian Bogor.
[10] Swift, S., M.J. Lynch., L. Fish., D.F. Leink., J.M. Thomas., C.J.A.B. Stewart and Williams P. 1999. Quorum Sensing-Dependen Regulation and Blokade of Kaseinase Production in Aeromonas hydrophila. Infection and Iminity. 4:18-28.
[11] Manefield, M., T.B. Rasmussen., M. Henzter., J.B. Andersen., S.P. Teinberg., S. Kjelleberg., and M. Givskov. 2000. Halogenated Furanones Inhibit Quorum Sensing Through Accelerated Luxr Turn Over. Microbiology. 148: 1119-1127.
[12] Peters, L., G.M. Konig., A.D. Wright., R. Pukall., E. Stakebrandt., L. Eberl and K. Riedel. 2003. Secondary Metabolites Flustra Foliacea and Their Influence on Bacteria. Applied and Environmental Microbiology: 3469-3475.
[13] Novitasari, Y., A. Pangastuti and R. Rakhmawati. 2014. Penghambatan Produksi Enzim Kaseinase Pada Sistem Quorum Sensing Aeromonas hydrophila Dengan Pemberian Ekstrak Methanol Rimpang Segar dan Rimpang Kering Lengkuas (Alpinia galanga). Jurusan Biologi. 12(2): 51-61.
[14] Hentzer, M and M. Givskov. 2003. Pharmalogical Inhibition of Quorum Sensing for The Treatment of Chronic Bacterial Infection. Clint. Invest. 112: 1300-1307.
[15] Pande, G.S.J., F.M.I. Natrah., P. Sorgeloos., P. Bossier and T. Defoirdt. 2013. Quorum Sensing Disrupting Compounds Protect Larvae of the Giant Freshwater Prawn Macrobrachium Rosenbergii from Vibrio Harveyi Infection. Aqua. Res. 43: 406-407.
[16] Yusriah and N.D. Kuswytasari, 2013. Pengaruh Ph Dan Suhu Terhadap Aktivitas Protease Penicillum sp. Jurnal Sains dan Seni Pomits. 2(1): 2337-3520.
[17] Kirov, S.M., M. Castrisios and J.G. Shaw. 2004. Aeromonas Flagella (Polar and Lateral) Are Enterocyte Adhesins That Contribute to Biofilm Formation on Surfaces. Infect. Immun. 72:1939-1945.
How to Cite
SINAGA, Ayu Ashari Margareth; JULYANTOTRO, Pande Gde Sasmita; PEBRIANI, Dewa Ayu Angga. Effect of Thiophenone Compound on Motility and Caseinase Production of Aeromonas hydrophila. Advances in Tropical Biodiversity and Environmental Sciences, [S.l.], v. 5, n. 2, p. 40-44, aug. 2021. ISSN 2622-0628. Available at: <https://ojs.unud.ac.id/index.php/ATBES/article/view/69722>. Date accessed: 19 jan. 2022. doi: https://doi.org/10.24843/ATBES.2021.v05.i02.p01.