Total Genomic DNA Extraction Studies from Seaweeds

  • Made Pharmawati Udayana University
  • Wildan Mujahidul Basyar Udayana University
  • Ida Ayu Astarini Udayana University


One of marine resources that has high value is seaweed.  Seaweed is a carrageenan producer used in the food industry. Seaweed contains many minerals, vitamins and proteins that are useful for health. Carotenoids are pigments found in seaweed that function as antioxidants.   The genes involved in carotenoid biosynthesis have been studied and provide opportunities for genetic improvement of seaweed.  DNA is a basic requirement in molecular analysis. Therefore, a suitable method of DNA extraction from seaweed is needed.  The aim of this research was to investigate DNA extraction method from several seaweed species and test the DNA quality through PCR-RAPD.  Seaweed samples were collected from Pantai Bumi Perkemahan Taman Nasional Bali Barat and DNA was extracted using Doyle and Doyle’s method with modifications.  PCR-RAPD was conducted using primer UBC127 and OPD 11 to test the quality of DNA.  Results showed that 3 hours incubation in 60ºC had the best result of DNA extraction.  However, the quality of DNA was low, as indicated by inconsistent PCR-RAPD products.   Further optimization in DNA extraction is needed to obtain high quality DNA for genetic analysis.


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Author Biographies

Made Pharmawati, Udayana University

Biology Study Program, Faculty of Mathematics and Natural Sciences

Wildan Mujahidul Basyar, Udayana University

Biology Study Program, Faculty of Mathematics and Natural Sciences

Ida Ayu Astarini, Udayana University

Biology Study Program, Faculty of Mathematics and Natural Sciences


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How to Cite
PHARMAWATI, Made; BASYAR, Wildan Mujahidul; ASTARINI, Ida Ayu. Total Genomic DNA Extraction Studies from Seaweeds. Advances in Tropical Biodiversity and Environmental Sciences, [S.l.], v. 4, n. 1, p. 10-14, mar. 2020. ISSN 2622-0628. Available at: <>. Date accessed: 04 july 2020. doi: