ISOLASI, IDENTIFIKASI DAN UJI POTENSI BAKTERI YANG BERPERAN PADA PENGOLAHAN AIR LIMBAH YANG MENGANDUNG RHODAMIN B DALAM BIOSISTEM TANAMAN

Abstract

Rhodamin B is a dangerous synthetic dyes substances used as a fabric dye in the
textile industry. Biodegradation is one way that is used in the processing of liquid waste
Rhodamin B. Biodegradation technique is leveraging the ability of microbial activities
degrade or eliminate pollutant compounds. The main objective of this research was to
investigate the characteristic of bacteria that are capable of living on wastewater containing
Rhodamin B and the effectiveness of single and microbial consortium isolates to degrade
Rhodamin B on wastewater. The isolation of the bacteria was done by plating method and the
identification of the bacteria by using a Kit API 20E. Test of bacterial potential of Rhodamin
B dyes substance carried out in the Microbiology Laboratory, Biology Department, Faculty
of Science, Udayana University. It was found in this research that five bacterial isolates were
obtained (Pseudomonas sp., Shigella spp., Stenotrophomonas sp., Pasteurella sp. dan Proteus
sp.). Pasteurella sp. had the highest percentage degrade effectiveness of 40.55%. Microbial
potential degrade Rhodamin B by Pasteurella sp. dan Proteus sp. results showed significant
differences (P <0.05) to control.
Keyword: bacteria, biodegradation, Rhodamin B