Keutuhan Membran Spermatozoa Disekuensing Sentrifugasi Gradien Densitas Percoll Berpengencer Andromed dan CEP-2 yang Ditambahkan Kuning Telur (MEMBRANE INTACT OF SPERMATOZOA FOLLOWING SEXING USING PERCOLL DENSITY GRADIENT CENTRIFUGATION IN ANDROMED AND C

  • Yudha Fika Diliyana Bagian Klinik Hewan, Fakultas Kedokteran Hewan, Universitas Udayana, Bali
  • Trinil Susilawati
  • Sri Rahayu

Abstract

The aim of this study was to observe the best extender in protecting the membrane of bovine spermatozoafollowing sexing by percoll density gradient centrifugation. Freshly collected semen were obtained fromBalai Besar Inseminasi Buatan Singosari-Malang. The semen were diluted in andromed and CaudalEpididymal Plasma-2 (CEP-2) added with 10% egg yolk extenders.The sperm membrane integrity wasobserved using Hypo-osmotic Swelling Test (HOST). Sperm capacitation and acrososome reaction wereassessed using Chlortetracycline Fluorescence Assay.The results showed that andromed and CEP-2 addedwith 10% egg yolk were able to retain the sperm membrane integrity, whereas sperm capacitation andacrosome reaction were kept low. Caudal Epididymal Plasma-2 (CEP-2) added with 10% egg yolk seemedto give better protection towards the sperm membrane intact in comparison to andromed extender.

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Published
2014-05-07
How to Cite
DILIYANA, Yudha Fika; SUSILAWATI, Trinil; RAHAYU, Sri. Keutuhan Membran Spermatozoa Disekuensing Sentrifugasi Gradien Densitas Percoll Berpengencer Andromed dan CEP-2 yang Ditambahkan Kuning Telur (MEMBRANE INTACT OF SPERMATOZOA FOLLOWING SEXING USING PERCOLL DENSITY GRADIENT CENTRIFUGATION IN ANDROMED AND C. Jurnal Veteriner, [S.l.], v. 15, n. 1, p. 23-30, may 2014. ISSN 2477-5665. Available at: <http://ojs.unud.ac.id/index.php/jvet/article/view/8781>. Date accessed: 12 sep. 2025.
Citation Formats
Issue
Vol 15 No 1 (2014)
Section
Articles

Keywords

andromed, Caudal Epididymal Plasma-2, sexing, spem membrane
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This work is licensed under a Creative Commons Attribution 4.0 International License
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