Deteksi Protein Bovine Major Histocompatibility Complex Klas I dan Klas II pada Sapi Madura

  • Ni Ketut Suwiti
  • Fedik Abdul Rantam
  • I Nengah Kerta Besung

Abstract

A study to detect the presence of bovine major histocompatibility complex (Bo-MHC) in maduracattle has been carried out. Lymphocyte samples were collected from 25 cattle. The protein of lymphocytesamples was analysed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) andtransferred onto nitrocellulose membrane. Bo-MHC proteins on the membrane were detected by monoclonalantibodies (anti-Bo-MHC class I) and BAQ150A (anti-Bo-MHC class II). The proteins of 48 kDa and 11kDa were detected by MAb B5C which appeared to be the alpha and beta chain of Bo-MHC class I. MAbBAQ150A detected protein band of 26 KDa which was likely to be the beta chain of Bo-MHC class II. Bo-MHC class I dan class II were clearly detected in madura cattle by MAbs which provides a basis for furtherinvestigation on the role of the MHC in the susceptibility the animals to many diseases.

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Author Biographies

Ni Ketut Suwiti
Laboratorium Histologi , 3Laboratorium BakteriologiFakultas Kedokteran Hewan, Universitas UdayanaJl. PB. Sudirman, Denpasar, Bali,Telepon: (0361) 223791, 701808;
Fedik Abdul Rantam
Laboratorium Virologi, Fakultas Kedokteran Hewan, Universitas AirlanggaKampus C Unair, Jl. Mulyorejo, Surabaya 60115
I Nengah Kerta Besung
Laboratorium Virologi, Fakultas Kedokteran Hewan, Universitas AirlanggaKampus C Unair, Jl. Mulyorejo, Surabaya 60115
How to Cite
SUWITI, Ni Ketut; ABDUL RANTAM, Fedik; KERTA BESUNG, I Nengah. Deteksi Protein Bovine Major Histocompatibility Complex Klas I dan Klas II pada Sapi Madura. Jurnal Veteriner, [S.l.], v. 9, n. 3, sep. 2008. ISSN 2477-5665. Available at: <http://ojs.unud.ac.id/index.php/jvet/article/view/3328>. Date accessed: 27 sep. 2025.
Citation Formats
Issue
Vol 9 No 3 (2008)
Section
Articles

Keywords

Bovine Major Histocompatibility Complex, madura cattle, monoclonal, antibodies
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License
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