AMINO-TERMINUS OF POLYMERASE BASIC-2 OF AVIAN INFLUENZA VIRUS OF H5N1 SUBTYPE ISOLATED FROM VARIOUS ANIMAL SPECIES IN INDONESIA

  • Gusti Ayu Yuniati Kencana
  • Widya Asmara
  • Charles Rangga Tabbu
  • I Gusti Ngurah Kade Mahardika

Abstract

The information on pathogenicity and adaptation factors of avian influenza virus (AIV) in mammalsis very inportant in an effort to reduce the risk of avian influenza (AI) pandemic in the future. Polymerasegene complex appears to be the major factors for adaptation of AIV to certain animal species. A preliminarystudy on role of non-coding region (NCR) and amino-terminus of polymerase-basic 2 (PB2) is presented.Purified viral RNA of AIV isolated from chicken, duck, pig, and quail of Bali and Yogyakarta was reversetranscribed into cDNA and amplified using reverse transcriptase-polymerase chain reaction (RT-PCR)using PB-2 universal forward primer and specifically designed backward primer. The result showed thatall AIV’s H5N1 isolated from chicken, duck, quail, and pig, posed PB2 amino-terminus typical for IndonesianAIV H5N1. However, polymorphic amino acids of the protein fragment did not show any species specificmotive, with the exception of the pig isolate Sw/Tabanan/2006 which had specific substitution of D16E,H17Q, M40I, and H124Y.

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Author Biographies

Gusti Ayu Yuniati Kencana
Laboratorium Virologi, Fakultas Kedokteran Hewan, Universitas Udayana,Jl. PB. Sudirman, Denpasar, Bali, Telepon: (0361) 223791
Widya Asmara
Laboratorium Mikrobiologi, Fakultas Kedokteran Hewan, Universitas Gadjah Mada,Jl. Olahraga Karang Malang,Yogyakarta
Charles Rangga Tabbu
Laboratorium Mikrobiologi, Fakultas Kedokteran Hewan, Universitas Gadjah Mada,Jl. Olahraga Karang Malang,Yogyakarta
I Gusti Ngurah Kade Mahardika
Laboratorium Biomedik dan Biologi Molekuler Hewan FKH Universitas Udayana,Jl.Raya Sesetan Gang Markisa No. 6 Denpasar, Bali
How to Cite
YUNIATI KENCANA, Gusti Ayu et al. AMINO-TERMINUS OF POLYMERASE BASIC-2 OF AVIAN INFLUENZA VIRUS OF H5N1 SUBTYPE ISOLATED FROM VARIOUS ANIMAL SPECIES IN INDONESIA. Jurnal Veteriner, [S.l.], v. 9, n. 3, sep. 2008. ISSN 2477-5665. Available at: <http://ojs.unud.ac.id/index.php/jvet/article/view/3323>. Date accessed: 27 sep. 2025.
Citation Formats
Issue
Vol 9 No 3 (2008)
Section
Articles

Keywords

PB2 gene, avian influenza, H5N1, Indonesia
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License
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