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PENENTUAN KUANTITATIF MORFIN DALAM URIN SECARA SPEKTROFOTODENSITOMETRI

N. M. Suaniti, M. A. Hitapretiwi Suryadhi

Abstract


Quantitative determination of morphine in urine has been done by utilizing spectrophotodensitometry.
Morphine standard in methanol 5 g/mL was spotted by linomat between 4-24 L in metal sheet thin layer
chromatography spectrophotodensito-metry using morphine standard of 20.40.60.80.100.120. and 140 ng.
Methode. statistically and validation analysis, showed a regression line of y = -69.21 + 8.06 x with r =
0.992. Detection and quantitation limits of 18.02 ng and 60.06 ng respectively were measured at maximum wave
length of (maks) 287 nm with on recovery of 90.91 percent.

Keywords


morphine, methanol, maximum wave length (maks), thin layer chromatography spectrophotodensitometry

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