Uji Sensitivitas Bakteri Klebsiella spp. yang Diisolasi dari Anjing Kintamani Diare Terhadap Antibiotika
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https://doi.org/10.24843/bulvet.2023.v15.i02.p07
Abstrak
Penelitian ini bertujuan untuk mengetahui pola kepekaan bakteri Klebsiella spp. yang diisolasi dari anjing kintamani diare. Sampel penelitian ini adalah 4 isolat Klebsiella spp. yang diisolasi dari anjing kintamani diare. Metode yang digunakan dalam penelitian ini yaitu metode difusi cakram Kirby-Bauer secara duplo dengan menggunakan antibiotika Kanamisin, Sulfamethoxazole-trimethoprim, Streptomisin. Data yang diperoleh dinyatakan secara deskriptif kualitiatif dengan mengukur zona hambat antibiotika yang terbentuk. Hasil penelitian menunjukkan bahwa islolat Klebsiella spp. 75% intermediet dan 25% sensitif terhadap Kanamisin, seluruh sampel sensitif terhadap Sulfamethoxazole-trimethoprim dan 75% sampel intermediet dan 25% sensitif terhadap Streptomisin
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Referensi
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Duse A, Persson-Waller K, Pedersen K. 2021. Microbial aetiology, antibiotic susceptibility and pathogen specific risk factors for udder pathogens from clinical mastitis in dairy cows. Animals. 11(7): 1-17.
Etebu E, Arikekpar I. 2016. Antibiotics: Classification and mechanisms of action with emphasis on molecular perspectives. Int. J. Appl. Microbiol. Biotechnol. Res. 4: 90-101.
Evayana M, Dwinata IM, Puja IK. 2017. Prevalensi infeksi cacing toxocara canis pada anjing kintamani di Desa Sukawana, Kecamatan Kintamani, Kabupaten Bangli, Bali. Indon. Med. Vet. 6(2): 115-123.
German AJ, Halladay LJ, Noble PJM. (2010). First-choice therapy for dogs presenting with diarrhoea in clinical practice. Vet. Rec. 167(21): 810–814.
Gómez-Beltrán DA, Schaeffer DJ, Ferguson DC, Monsalve LK, Villar D. 2021. Antimicrobial prescribing practices in dogs and cats by colombian veterinarians in the city of medellin. Vet. Sci. 8(5): 1-11.
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Hossain S, De Silva BCJ, Dahanayake PS, Heo G. 2019. Phylogenetic relationships, virulence and antimicrobial resistance properties of Klebsiella spp. isolated from pet turtles in Korea. Let. Appl. Microbiol. 70: 71-78.
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Mustika OC, Pinatih KJP, Suardana IW. 2015. Uji kepekaan Escherichia coli O157:H7 feses sapi di Kecamatan Kuta Selatan Badung Bali terhadap antibiotik. Indon. Med. Vet. 4(4): 342-350.
Nazara AL, Suarjana IGK, Tono KPG. 2022. Bakteri klebsiella sp. asal babi penderita porcine respiratory disease complex resistan terhadap ampicillin dan peka sulfamethoxazole-trimethoprim dan kanamycin. Indon. Med. Vet. 11(1): 66-75.
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Puja IK. 2007. Anjing kintamani maskot fauna Kabupaten Bangli. Penerbit Universitas Udayana Bali.
Purba DA, Tono KPG, Suarjana IGK. 2022. Uji kepekaan streptococcus spp.yang diisolasi dari penyakit saluran pernapasan kompleks babi terhadap kanamycin, streptomycin dan doxyxycline. Bul. Vet. Udayana. 14(3): 202-209.
Samanta I, Bandyopadhyay S. 2020. Klebsiella. Antimicrobial Resistance in Agriculture. Elsevier Pp. 153–169.
Santosa PE. 2016. Efektivitas berbagai preparat antibiotika terhadap kasus omphalitis pada ayam broiler. J. Ilmiah Pet. Terpadu. 4(4): 319 – 322.
Sharif NM, Sreedevi B, Chaitanya RK, Sreenivasulu D. 2017. Beta-lactamase antimicrobial resistance in Klebsiella and Enterobacter species isolated from healthy and diarrheic dogs in Andhra Pradesh, India. Vet. World. 10(8): 950-954.
Shi Y, Yang H, Chu M, Niu X, Huo X, Gao Y, Zeng J, Zhang T, Li YG, Outi K, Lou K, Li X, Dang, Li C. 2020. Klebsiella. Beneficial Microbes in Agro-Ecol. Pp. 233–257.
Upmanyu N, Malviya VN. (2020). Antibiotics: mechanisms of action and modern challenges. Microorganisms for Sustainable Environment and Health. Pp. 367–382.
Widyasari AM, Misyetti TH, Ambar, Nuraeni. 2013. Karakteristik fisikokimia kit kering kanamycin. J. Sains Teknol. Nuklir Indon. 43(2): 117-126.
Zhang X, Wang J, Wu Q, Li L, Wang Y, Yang H. (2019). Determination of kanamycin by high performance liquid chromatography. Molecules. 24(10): 1902.
Anholt RM, Berezowski J, Ribble CS, Russell ML, Stephen C. 2014. Using informatics and the electronic medical record to describe antimicrobial use in the clinical management of diarrhea cases at 12 companion animal practices. PLoS One. 9(7): e103190.
Clinical Laboratory Standards Institute (CLSI). 2020. Performance standards for antimicrobial susceptibility testing. 30th ed. CLSI supplement M100. Wayne, PA: Clinical and Laboratory Standards Institute
Dewandaru RA, Indarjulianto S, Yanuartono, Nururrozi A, Purnamaningsih A, Hayati R. 2019. Diare disebabkan infeksi escherichia coli pada anjing. J. Trop. Anim. Vet. Sci. 9(2): 38-43.
Duse A, Persson-Waller K, Pedersen K. 2021. Microbial aetiology, antibiotic susceptibility and pathogen specific risk factors for udder pathogens from clinical mastitis in dairy cows. Animals. 11(7): 1-17.
Etebu E, Arikekpar I. 2016. Antibiotics: Classification and mechanisms of action with emphasis on molecular perspectives. Int. J. Appl. Microbiol. Biotechnol. Res. 4: 90-101.
Evayana M, Dwinata IM, Puja IK. 2017. Prevalensi infeksi cacing toxocara canis pada anjing kintamani di Desa Sukawana, Kecamatan Kintamani, Kabupaten Bangli, Bali. Indon. Med. Vet. 6(2): 115-123.
German AJ, Halladay LJ, Noble PJM. (2010). First-choice therapy for dogs presenting with diarrhoea in clinical practice. Vet. Rec. 167(21): 810–814.
Gómez-Beltrán DA, Schaeffer DJ, Ferguson DC, Monsalve LK, Villar D. 2021. Antimicrobial prescribing practices in dogs and cats by colombian veterinarians in the city of medellin. Vet. Sci. 8(5): 1-11.
Grosset JH, Singer T. (2013). Streptomycin. Brenner’s Encyclopedia of Genetics. 5: 568–569.
Hansur L, Ugi D, Hamball H. 2019. Uji kepekaan bakteri asam laktat kandidat probiotik terhadap antibiotik kanamisin, oleandomisin, dan polimiksin B. eJKI. 7(1): 61-65.
Hossain S, De Silva BCJ, Dahanayake PS, Heo G. 2019. Phylogenetic relationships, virulence and antimicrobial resistance properties of Klebsiella spp. isolated from pet turtles in Korea. Let. Appl. Microbiol. 70: 71-78.
Koneman EW, Procop GW, Church DL, Hall GS, Janda WM, Woods GL. 2017. Koneman's color atlas and textbook of diagnostic microbiology. Philadelphia: Lippincott Williams & Wilkins.
Krause KM, Serio AW, Kane TR, Connolly LE. (2016). Aminoglycosides: an overview. Cold Spring Harbor Perspectives in Med. 6(6): 1-18.
Majewski M, Józefiak A, Kimsa-Furdzik M, Dziubdziela L, Hudak-Nowak M, Wilczyński J, Anusz K. 2021. Antimicrobial resistance of Escherichia coli and Klebsiella spp. conventionally sampled from factory-farmed chickens clinical submissions. Ann. Agric. Environ. Med. 28(2): 271–276.
Marks SL, Kather EJ. 2003. Bacterial-associated diarrhea in the dog: a critical appraisal. Small Anim. Pract. 33(5): 1029–1060.
Marks SL, Rankin SC, Byrne BA, Wesse, JS. 2011. Enteropathogenic bacteria in dogs and cats: diagnosis, epidemiology, treatment, and control. J. Vet. Intern. Med. 25(6):1195–1209.
Munirah, Rasidah E, Melani N, Zakiah, Nasir M. 2018. Uji aktivitas antibakteri etanol daun ketapang (terminalia catappa l.) warna hijau dan warna merah serta kombinasinya. Indon. J. Pharm. Nat. Prod. 1(2): 8-13.
Mustika OC, Pinatih KJP, Suardana IW. 2015. Uji kepekaan Escherichia coli O157:H7 feses sapi di Kecamatan Kuta Selatan Badung Bali terhadap antibiotik. Indon. Med. Vet. 4(4): 342-350.
Nazara AL, Suarjana IGK, Tono KPG. 2022. Bakteri klebsiella sp. asal babi penderita porcine respiratory disease complex resistan terhadap ampicillin dan peka sulfamethoxazole-trimethoprim dan kanamycin. Indon. Med. Vet. 11(1): 66-75.
Pilla R, Suchodolski JS. 2019. The role of the canine gut microbiome and metabolome in health and gastrointestinal disease. Front. Vet. Sci. 6(498): 1-12.
Puja IK. 2007. Anjing kintamani maskot fauna Kabupaten Bangli. Penerbit Universitas Udayana Bali.
Purba DA, Tono KPG, Suarjana IGK. 2022. Uji kepekaan streptococcus spp.yang diisolasi dari penyakit saluran pernapasan kompleks babi terhadap kanamycin, streptomycin dan doxyxycline. Bul. Vet. Udayana. 14(3): 202-209.
Samanta I, Bandyopadhyay S. 2020. Klebsiella. Antimicrobial Resistance in Agriculture. Elsevier Pp. 153–169.
Santosa PE. 2016. Efektivitas berbagai preparat antibiotika terhadap kasus omphalitis pada ayam broiler. J. Ilmiah Pet. Terpadu. 4(4): 319 – 322.
Sharif NM, Sreedevi B, Chaitanya RK, Sreenivasulu D. 2017. Beta-lactamase antimicrobial resistance in Klebsiella and Enterobacter species isolated from healthy and diarrheic dogs in Andhra Pradesh, India. Vet. World. 10(8): 950-954.
Shi Y, Yang H, Chu M, Niu X, Huo X, Gao Y, Zeng J, Zhang T, Li YG, Outi K, Lou K, Li X, Dang, Li C. 2020. Klebsiella. Beneficial Microbes in Agro-Ecol. Pp. 233–257.
Upmanyu N, Malviya VN. (2020). Antibiotics: mechanisms of action and modern challenges. Microorganisms for Sustainable Environment and Health. Pp. 367–382.
Widyasari AM, Misyetti TH, Ambar, Nuraeni. 2013. Karakteristik fisikokimia kit kering kanamycin. J. Sains Teknol. Nuklir Indon. 43(2): 117-126.
Zhang X, Wang J, Wu Q, Li L, Wang Y, Yang H. (2019). Determination of kanamycin by high performance liquid chromatography. Molecules. 24(10): 1902.
Diterbitkan
2023-03-06
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ANANTA KUSUMA, Anak Agung Gede Agung; SUARJANA, I Gusti Ketut; GELGEL, Ketut Tono Pasek.
Uji Sensitivitas Bakteri Klebsiella spp. yang Diisolasi dari Anjing Kintamani Diare Terhadap Antibiotika.
Buletin Veteriner Udayana, [S.l.], p. 205-210, mar. 2023.
ISSN 2477-2712.
Tersedia pada: <http://ojs.unud.ac.id/index.php/buletinvet/article/view/86801>. Tanggal Akses: 20 feb. 2026
doi: https://doi.org/10.24843/bulvet.2023.v15.i02.p07.
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