Gambaran Patologi Anatomi dan Histopatologi Organ Pertahanan Itik Bali Pascainfeksi Buatan Avian orthoavulavirus 1 Isolat Tabanan-1/ARP/2017
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https://doi.org/10.24843/bulvet.2022.v14.i06.p08
Abstrak
Penelitian ini bertujuan untuk mengetahui perubahan patologi anatomi dan histopatologi organ timus, bursa Fabricius, dan limpa itik bali yang diinfeksi virus newcastle disease atau Avian orthoavulavirus 1 isolat Tabanan-1/ARP/2017. Penelitian ini menggunakan 15 ekor itik bali jantan berumur satu minggu. Setelah periode aklimatisasi selama dua minggu dan pengukuran titer antibodi ND dalam serum itik menunjukkan hasil di bawah angka 23 HI unit, selanjutnya sejumlah 10 ekor itik diinfeksikan dengan AOAV-1 isolat Tabanan-1/ARP/2017 dengan dosis 0,5ml/27 HA unit secara intraokular (P1) dan 5 ekor itik lainnya diberikan 0,5 ml larutan phosphate buffered saline melalui rute yang sama sebagai kontrol (P0). Nekropsi kemudian dilakukan pada itik yang mati dan dilakukan pemeriksaan patologi anatomi terhadap organ pertahanannya. Selanjutnya organ tersebut diproses dan dipulas dengan pewarnaan Hematoksilin-Eosin, lalu dilakukan pemeriksaan histopatologi. Hasil penelitian menunjukkan bahwa infeksi AOAV-1 isolat Tabanan-1/ARP/2017 pada itik bali dapat menyebabkan perubahan patologi anatomi berupa lesi foki nekrotik pada limpa dan atrofi pada timus. Sementara pada gambaran histopatologi menimbulkan deplesi sel limfoid berderajat parah pada organ timus dan bursa Fabricius, serta deplesi sel limfoid berderajat ringan pada organ limpa.
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Referensi
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Kang Y, Feng M, Zhao X, Dai X, Xiang B, Gao P, Li Y, Li Y, Ren T. 2016. Newcastle disease virus infection in chicken embryonic fbroblasts but not duck embryonic fbroblasts is associated with elevated host innate immune response. Virol. J. 13: 41.
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Rohajawati S, Supriyati R. 2010. Diagnosis penyakit unggas dengan metode certainty factor. Comm. IT. 4(1): 41-46.
Roy P, Venugopalan AT, Manvell R. 2000. Characterization of Newcastle disease virus isolated from chickens and ducks in Tamilnadu, India. Vet. Res. Comm. 24: 135-142.
Rue CA, Susta L, Cornax I, Brown CC, Kapczynski DR, Suarez DL, King DJ, Miller PJ, Afonso CL. 2011. Virulent Newcastle disease virus elicits a strong innate immune response in chickens. J. Gen. Virol. 92: 931-939.
Saepulloh M, Darminto. 2005. Kajian newcastle disease pada itik dan upaya pengendaliannya. WARTAZOA. 15(2): 84-94.
Sultan S, Osman N, Mohamed MA, Ahmed AI, Abdallah RAM, Faraouk M, Taha M, 2016. Infectious bursal disease vaccine ameliorates velogenic newcastle disease virus infection in immunopotentiated chickens. Comp. Clin. Pathol. 25(1): 91-100.
Udayana IDGA. 2012. Itik Bali Sebagai Hewan Upacara dan Produksi. Denpasar. Udayana University Press.
Zachary JF. 2012. Nervous System. In: McGavin MD, Zachary JF. (Ed.) Pathologic Basis of Veterinary Disease. 5th ed. St. Louis (US). Mosby Elsivier. Pp. 771-870.
Zahid B, Akhtar R, Ahmed QA, Akram Q, Yasmeen R, Ateeq MK, Raza M, Rizwan HM, Iqbal Z, Saleem MM, Imran M. 2021. Clinico-pathological assessment of virulent Newcastle Disease Virus in ducks. Braz. J. Biol. 84.
Adi AAAM, Astawa NM, Putra KSA, Hayashi Y, Matsumoto Y. 2010. Isolation and characterization of a pathogenic newcastle disease virus from a natural case in Indonesia. J. Vet. Med. Sci. 72: 313-319.
Adi AAAM, Astawa NM, Wandia IN, Putra IGAA, Winaya IBO, Krisnandika AAK, Wijaya AAGO. 2019b. Karakteristik molekuler virus avian orthoavulavirus 1 genotipe VII yang diisolasi dari Tabanan Bali. J Vet. 20(4): 593-602.
Aguanta BN, Fuller AL, Milfort MC, Williams SM, Rekaya R, Aggrey SE. 2018. Histologic Effects of Concurrent Heat Stress and Coccidial Infection on the Lymphoid Tissues of Broiler Chickens. Avian Dis. 62: 345–350.
Anis Z, Morita T, Azuma K, Ito H, Itoand T, Shimada A. 2013. Histopathological alterations in immune organs of chickens and ducks after experimental infection with virulent 9a5b newcastle disease virus. J. Comp. Path. 149: 82-93.
Berthault C, Larcher T, Härtle S, Vautherot JF, Trapp-Fragnet L, Denesvre C. 2018. Atrophy of primary lymphoid organs induced by Marek’s disease virus during early infection is associated with increased apoptosis, inhibition of cell proliferation and a severe B-lymphopenia. Vet. Res. 49(1).
Bharathi AA, Muthusamy P, Rathnapraba S, Selvan ST, Srinivasan G. 2018. Evaluation of histopathological changes in ducks infected with newcastle disease virus. Int. J. Curr. Microbiol. App. Sci. 7(6): 3768-3774.
Dai Y, Cheng X, Liu M, Shen X, Li J, Yu S, Zou J, Ding C. 2014. Experimental infection of duck origin virulent Newcastle disease virus strain in duck. BMC Vet. Res. 10: 164.
Elbestawy AR, Ellakany HF, El-Hamid HSA, Zedan RE, Gado AR, Sedeik ME, El-Hack MEA, Saadeldin IM, Alowaimer AN, Ba-Awadh H, Swelum AA. 2019. Muscovy ducks infected with velogenic Newcastle disease virus (genotype VIId) act as carriers to infect in-contact chickens. Poult. Sci. 10: 1–8.
Etriwati, Ratih D, Handharyani E, Setyaningsinh S. 2017. Pathology and immunohistochemistry study of Newcastle disease field case in chicken in Indonesia. Vet. World. 10(9): 1066-1071.
Harrison L, Brown C, Afonso C, Zhang J, Susta L. 2011. Early occurrence of apoptosis in lymphoid tissues from chickens infected with strains of newcastle disease virus of varying virulence. J. Comp. Pathol. 145(4): 327–335.
Kang Y, Feng M, Zhao X, Dai X, Xiang B, Gao P, Li Y, Li Y, Ren T. 2016. Newcastle disease virus infection in chicken embryonic fbroblasts but not duck embryonic fbroblasts is associated with elevated host innate immune response. Virol. J. 13: 41.
Kiernan JA. 2015. Histological and Histochemical Methods: Theory and Practice. 5th Ed. United Kingdom. Scion Publishing. Pp. 330-334.
Kingston DJ, Dharsana R, Chavez ER. 1978. Isolation of mesogenic Newcastle disease virus from an acute disease in Indonesian ducks. Trop. Anim. Health. Prod. 10: 161-164.
Lu A, Diao Y, Chen H, Wang J, Ge P, Sun X, Hao D. 2014. Evaluation of histopathological changes, viral load, and immune function of domestic geese infected with Newcastle disease Virus. Avian Pathol. 43: 325–332.
Ravindra PV, Tiwari AK, Ratta B, Bais MV, Chaturvedi U, Palia SK, Sharma B, Chauhan RS. 2009. Time course of newcastle disease virus induced apoptotic pathways. Virus Res. 144: 350–54.
Rohajawati S, Supriyati R. 2010. Diagnosis penyakit unggas dengan metode certainty factor. Comm. IT. 4(1): 41-46.
Roy P, Venugopalan AT, Manvell R. 2000. Characterization of Newcastle disease virus isolated from chickens and ducks in Tamilnadu, India. Vet. Res. Comm. 24: 135-142.
Rue CA, Susta L, Cornax I, Brown CC, Kapczynski DR, Suarez DL, King DJ, Miller PJ, Afonso CL. 2011. Virulent Newcastle disease virus elicits a strong innate immune response in chickens. J. Gen. Virol. 92: 931-939.
Saepulloh M, Darminto. 2005. Kajian newcastle disease pada itik dan upaya pengendaliannya. WARTAZOA. 15(2): 84-94.
Sultan S, Osman N, Mohamed MA, Ahmed AI, Abdallah RAM, Faraouk M, Taha M, 2016. Infectious bursal disease vaccine ameliorates velogenic newcastle disease virus infection in immunopotentiated chickens. Comp. Clin. Pathol. 25(1): 91-100.
Udayana IDGA. 2012. Itik Bali Sebagai Hewan Upacara dan Produksi. Denpasar. Udayana University Press.
Zachary JF. 2012. Nervous System. In: McGavin MD, Zachary JF. (Ed.) Pathologic Basis of Veterinary Disease. 5th ed. St. Louis (US). Mosby Elsivier. Pp. 771-870.
Zahid B, Akhtar R, Ahmed QA, Akram Q, Yasmeen R, Ateeq MK, Raza M, Rizwan HM, Iqbal Z, Saleem MM, Imran M. 2021. Clinico-pathological assessment of virulent Newcastle Disease Virus in ducks. Braz. J. Biol. 84.
Diterbitkan
2022-05-12
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AL MA'ARIF, M. Farhan; ADI, Anak Agung Ayu Mirah; WINAYA, Ida Bagus Oka.
Gambaran Patologi Anatomi dan Histopatologi Organ Pertahanan Itik Bali Pascainfeksi Buatan Avian orthoavulavirus 1 Isolat Tabanan-1/ARP/2017.
Buletin Veteriner Udayana, [S.l.], p. 659-667, may 2022.
ISSN 2477-2712.
Tersedia pada: <http://ojs.unud.ac.id/index.php/buletinvet/article/view/86256>. Tanggal Akses: 20 feb. 2026
doi: https://doi.org/10.24843/bulvet.2022.v14.i06.p08.
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