PENENTUAN KINETIKA ENZIM POLIGALAKTURONASE (PG) ENDOGENOUS DARI PULP BIJI KAKAO

  • G.P. GANDA PUTRA

Abstract

This research was conducted to determine the enzyme kinetics (Km and Vmax) of endogenous polygalacturonase (PGs) isolated from cocoa pulp. This research was carried out by assaying PGs activities in various concentration of citrus pectin subtrates which were between 0,1 – 1,0% with 0,1% interval. The result showed that the enzyme kinetics of endogenous PGs from cocoa pulp (Km) was 0,37% and Vmax was 6,69 µmol of galacturonic acid/minute/ml.

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Author Biography

G.P. GANDA PUTRA
Jurusan Teknologi Industri Pertanian, Fakultas Teknologi Pertanian, Universitas Udayana, Kampus Bukit Jimbaran
Published
2009-06-01
How to Cite
GANDA PUTRA, G.P.. PENENTUAN KINETIKA ENZIM POLIGALAKTURONASE (PG) ENDOGENOUS DARI PULP BIJI KAKAO. Jurnal Biologi Udayana, [S.l.], v. 13, n. 1, p. -, june 2009. ISSN 2599-2856. Available at: <https://ojs.unud.ac.id/index.php/bio/article/view/575>. Date accessed: 16 dec. 2024.

Keywords

enzymatic kinetics (Km , Vmax); PGs; cocoa pulp